FastQCFastQC Report
Mon 23 Aug 2021
HGVMYDRXY_n01_CIVRCoV_P2_B08_Neg2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGVMYDRXY_n01_CIVRCoV_P2_B08_Neg2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28201
Sequences flagged as poor quality0
Sequence length151
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCGTAACATCTCGTAT316011.205276408637992TruSeq Adapter, Index 11 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCGTAACATCGCGTAT16285.7728449345767885TruSeq Adapter, Index 11 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCGTAACATCTCGTA4191.485762916208645TruSeq Adapter, Index 11 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCGTAACATCGCGTA2590.9184071486826708TruSeq Adapter, Index 11 (97% over 36bp)
ATAATTATAATTATAATTATAATTATAATTATAATTATAATTATAATTAT1530.5425339526967129No Hit
AATTATAATTATAATTATAATTATAATTATAATTATAATTATAATTATAA940.3333215134215099No Hit
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCGTAACATCTCGTA920.32622956632743516TruSeq Adapter, Index 4 (97% over 35bp)
ATTATAATTATAATTATAATTATAATTATAATTATAATTATAATTATAAT670.23758022765150172No Hit
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC590.209212439275203Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TAATTATAATTATAATTATAATTATAATTATAATTATAATTATAATTATA570.20212049218112832No Hit
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCGTAACATCGCGTA540.19148257154001633TruSeq Adapter, Index 4 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCGTAACATCTGGTAT500.17729867735186694TruSeq Adapter, Index 11 (97% over 36bp)
TATAATTATAATTATAATTATAATTATAATTATAATTATAATTATAATTA490.1737527038048296No Hit
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC450.15956880961668027Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG380.1347469947874189Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC360.1276550476933442Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GATCGGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCGTAA350.12410907414630687TruSeq Adapter, Index 11 (97% over 36bp)
ATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACT340.12056310059926953Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAA340.12056310059926953Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTTCCGATCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCGTAA300.10637920641112017TruSeq Adapter, Index 11 (97% over 36bp)
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTG290.10283323286408284TruSeq Adapter, Index 2 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)