FastQCFastQC Report
Mon 23 Aug 2021
HGVMYDRXY_n01_CIVRCoV_P1_E04_Neg1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGVMYDRXY_n01_CIVRCoV_P1_E04_Neg1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21165
Sequences flagged as poor quality0
Sequence length151
%GC62

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATCCGTATCTCGTAT373917.665957949444838TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATCCGTATCGCGTAT212310.03071107961257TruSeq Adapter, Index 7 (97% over 35bp)
ATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTC5822.749822820694543Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCAC5102.4096385542168677Illumina Multiplexing PCR Primer 2.01 (100% over 34bp)
ATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAG2931.3843609733049846Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATCCGTATCTCGTA2861.3512875029529885TruSeq Adapter, Index 7 (97% over 35bp)
ATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCC2731.2898653437278524Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAACT2321.0961493030947318Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATCCGTATCGCGTA2221.0489014883061658TruSeq Adapter, Index 7 (97% over 35bp)
ATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTGAA1970.9307819513347507Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATCCGTATCTCGGAT1360.642570281124498TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATCCGTATCGCGGAT1330.6283959366879281TruSeq Adapter, Index 7 (97% over 35bp)
ATATATATATATATATATATATATATATAGATCGGAAGAGCACACGTCTG1260.595322466335932Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATATATATATATATAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGA1120.5291755256319395TruSeq Adapter, Index 7 (97% over 35bp)
ATATATATATATATATATATATATATATATAGATCGGAAGAGCACACGTC960.45357902197023386No Hit
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATCCGTATCTCGTA920.4346798960548075TruSeq Adapter, Index 9 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATCCGTATCTCGTTT840.3968816442239546TruSeq Adapter, Index 7 (97% over 35bp)
ATATATATATATATATATATATATATATATATAGATCGGAAGAGCACACG700.3307347035199622No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATCCGTATCTGGTAT660.3118355776045358TruSeq Adapter, Index 7 (97% over 35bp)
TATATATATATATATATATATATATATATATATAAGATCGGAAGAGCACA630.297661233167966No Hit
TATATATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTG620.2929364516891094Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACT620.2929364516891094Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TATATATATATATATATATATATATATATATAAGATCGGAAGAGCACACG560.26458776281596974No Hit
TATATATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAA540.2551381998582566Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GAATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATCCGTATCGCGTA490.23151429246397354TruSeq Adapter, Index 9 (97% over 35bp)
ATATATATATATATATATATATATATATATATATAGATCGGAAGAGCACA480.22678951098511693No Hit
TATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCCAG450.21261516654854712Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
TATATATATATATATATATATATATATATAAGATCGGAAGAGCACACGTC420.1984408221119773No Hit
TATATATATATATATATATATATATATATATATATAAGATCGGAAGAGCA420.1984408221119773No Hit
TATATATATATATATATATATAAGATCGGAAGAGCACACGTCTGAACTCC390.1842664776754075Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATCCGTATCGCGTTT360.1700921332388377TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATCCGTATCGGGTAT340.1606425702811245TruSeq Adapter, Index 7 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATCCGTATCTCGTATG320.1511930073234113TruSeq Adapter, Index 7 (97% over 34bp)
TATATATATATATATATATATATATATATATATATATAAGATCGGAAGAG290.13701866288684147No Hit
GATCGGAAGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATCCG260.12284431845027168TruSeq Adapter, Index 7 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)