FastQCFastQC Report
Fri 31 Mar 2017
HGVKWBGX2_n01_sl40_grab_i24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGVKWBGX2_n01_sl40_grab_i24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15144299
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG9123186.024167906352087RNA PCR Primer, Index 24 (100% over 43bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG752300.4967545873202847RNA PCR Primer, Index 24 (100% over 43bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT1184100.066.3952249
TGCTTGA1183100.066.3788858
CATCTCG1169250.066.3523339
TATGCCG1191100.066.32493646
ATGCCGT1190450.066.3219447
TCTCGTA1178250.066.3199441
GCTTGAA1187050.066.3124159
GTATGCC1193100.066.302345
TGCCGTC1188450.066.2875348
CCGTCTT1187450.066.28097550
GCATCTC1165450.066.2708238
CGTATGC1194200.066.2645644
CTTGAAA1192250.066.2559560
ATCTCGT1179950.066.2347840
TAGCATC1163450.066.1722236
GTAGCAT1175100.066.0685635
GGTAGCA1177800.065.96104434
CGGTAGC1180150.065.8652133
CACGGTA1193750.065.827831
ACGGTAG1188900.065.818232
Produced by FastQC (version 0.11.2)