FastQCFastQC Report
Fri 31 Mar 2017
HGVKWBGX2_n01_sl40_grab_i23.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGVKWBGX2_n01_sl40_grab_i23.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15439145
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA10191876.601317624777797TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAG663050.4294603101402312TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCTTGA1299900.065.7751160
GCTTGAA1304500.065.7505461
TGGATAT1321950.065.6955137
CTTGAAA1310700.065.6894562
GCCGTCT1314600.065.62350551
GTATGCC1322800.065.6114147
TATGCCG1319750.065.6112248
CGTATGC1326000.065.59785546
TCTCGTA1323150.065.5944843
ATGCCGT1319800.065.5917149
ATCTCGT1325450.065.5513542
CCGTCTT1318550.065.54726452
TGCCGTC1318050.065.5378950
GAGTGGA1323500.065.4969134
TCACGAG1348850.065.2321330
TATCTCG1356600.063.7920941
TCTGAAC1410450.063.71696518
GAAAAAA1390400.063.70346565
CTGCTTG1345200.063.4265259
GGATATC1375950.063.20858438
Produced by FastQC (version 0.11.2)