FastQCFastQC Report
Fri 31 Mar 2017
HGVKWBGX2_n01_sl40_grab_i22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGVKWBGX2_n01_sl40_grab_i22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15037849
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA8972325.966491617251909TruSeq Adapter, Index 22 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAG574080.3817567259785625TruSeq Adapter, Index 22 (97% over 40bp)
CCCCCACCCACGAGGAGCATCGTGGAAAAAGAAGACGTTCCAACCACGTCTTCAAAGCAAGTGGATTGATGTGAT151120.1004930957878351No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTGAA1154500.066.0880661
CTTGAAA1161950.066.0714762
TGCTTGA1149600.066.03396660
AATCTCG1159050.066.02724541
TCTCGTA1166150.065.9210743
GCCGTCT1166750.065.8787451
ATCTCGT1166850.065.86442
ATGCCGT1175250.065.8394249
TATGCCG1175650.065.8341848
GTATGCC1181100.065.8199947
CGTATGC1182600.065.80804446
TGCCGTC1171600.065.7858550
CCGTCTT1171450.065.77093552
TACGTAA1184350.065.4898736
GTACGTA1186450.065.42586535
CGTACGT1184950.065.3631134
CCGTACG1192450.065.2568633
ACCGTAC1199300.065.17136432
CACCGTA1207600.064.9820931
TCACCGT1210750.064.83211530
Produced by FastQC (version 0.11.2)