FastQCFastQC Report
Fri 31 Mar 2017
HGVKWBGX2_n01_sl40_grab_i21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGVKWBGX2_n01_sl40_grab_i21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63154
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA24173.827152674414922TruSeq Adapter, Index 21 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTATGCCGTCTTCTGCGTGAAAAAAAAAA1930.3056021788010261TruSeq Adapter, Index 21 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTCCGCACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA1930.3056021788010261TruSeq Adapter, Index 21 (97% over 39bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1350.21376318206289385No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG1110.1757608385850461RNA PCR Primer, Index 17 (100% over 43bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTAACTTAGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG1010.1599265288026095TruSeq Adapter, Index 3 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA770.1219241853247617TruSeq Adapter, Index 20 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCCG301.1143675E-769.033
CGTTTCA150.002345227269.033
ACAGTCA150.002345227269.026
CGATATC700.069.038
GAGTGGA253.060677E-669.034
GTCGTTA150.002345227269.036
GCGATCT150.002345227269.037
GCGATAT150.002345227269.037
TTCCGCA253.060677E-669.035
GGCGTCT354.0763553E-969.051
CACGTGT401.4915713E-1069.031
ATCTAAT208.45215E-569.042
CATCTGA150.002345227269.016
TCGCGTG150.002345227269.043
TTATCGA150.002345227269.040
GTCGATA253.060677E-669.036
ACGTAGC301.1143675E-769.032
CACGAGA208.45215E-569.031
CACGAAT208.45215E-569.031
CGTAGCG253.060677E-669.033
Produced by FastQC (version 0.11.2)