FastQCFastQC Report
Fri 31 Mar 2017
HGVKWBGX2_n01_sl40_grab_i20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGVKWBGX2_n01_sl40_grab_i20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31924041
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA19058705.970014886273326TruSeq Adapter, Index 20 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAG1292210.40477645044999155TruSeq Adapter, Index 20 (97% over 44bp)
CCCCCACCCACGAGGAGCATCGTGGAAAAAGAAGACGTTCCAACCACGTCTTCAAAGCAAGTGGATTGATGTGAT337820.10581993676803009No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGAAA2443350.066.2606462
GCTTGAA2426550.066.247361
TGCTTGA2416450.066.16894560
TTATCTC2460800.066.08742540
GCCGTCT2460850.066.0774151
TCTCGTA2481550.066.0768343
ATCTCGT2488800.066.0497142
TATGCCG2478700.066.0485848
GTATGCC2493350.066.0246547
CGTATGC2492600.066.023346
ATGCCGT2479350.066.0195649
TGCCGTC2471950.065.9858450
CCGTCTT2471100.065.9786552
GGCCTTA2481200.065.886236
CACGTGG2532050.065.52328531
TCACGTG2539850.065.4839330
GTCACGT2561100.065.3849229
GAAAAAA2636250.064.0313765
TCTGAAC2684000.063.7281518
CTGCTTG2502050.063.67020859
Produced by FastQC (version 0.11.2)