FastQCFastQC Report
Fri 31 Mar 2017
HGVKWBGX2_n01_sl40_grab_i19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGVKWBGX2_n01_sl40_grab_i19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18669918
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA15549598.328686821227603TruSeq Adapter, Index 19 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAG947850.5076883572814835TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTGAA1989700.066.07950661
TGCTTGA1982050.066.076760
TCTCGTA1983000.066.05923543
ATCTCGT1966750.066.0289742
GCCGTCT1999250.065.9755351
AAACGAT1982450.065.97362537
CGTATGC2015800.065.94672446
TATGCCG2008250.065.94440548
ATGCCGT2007500.065.9443249
GTATGCC2013100.065.93655447
TGCCGTC2003250.065.9183750
CCGTCTT2006200.065.9173852
CTTGAAA2004750.065.9156962
GAAACGA1983350.065.7928936
TGAAACG1990250.065.7223535
GTGAAAC2000650.065.6567834
CACGTGA2027350.065.56756631
TCACGTG2031950.065.5143330
GTCACGT2045850.065.4141929
CGTGAAA2022800.065.338333
Produced by FastQC (version 0.11.2)