FastQCFastQC Report
Fri 31 Mar 2017
HGVKWBGX2_n01_sl40_grab_c12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGVKWBGX2_n01_sl40_grab_c12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11934435
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG10562968.850825363747845TruSeq Adapter, Index 12 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG937180.7852738734594473TruSeq Adapter, Index 12 (100% over 63bp)
CCCCCACCCACGAGGAGCATCGTGGAAAAAGAAGACGTTCCAACCACGTCTTCAAAGCAAGTGGATTGATGTGAT135780.11377161968706519No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTGAA1377900.066.6317159
TGCTTGA1376550.066.61767658
GCCGTCT1379500.066.57468449
TATGCCG1383900.066.53641546
TGCCGTC1381400.066.5327848
ATGCCGT1383600.066.5256347
GTATGCC1385900.066.5117545
CGTATGC1387300.066.50790444
TCTCGTA1364450.066.49769641
CCGTCTT1381650.066.489450
AATCTCG1359300.066.4786739
TGTAATC1342350.066.4353936
ATCTCGT1365450.066.4296440
CTTGAAA1387650.066.3602760
CTTGTAA1361750.065.94466434
AGTCACC1409000.065.67377528
CTGCTTG1402100.065.3465857
GAAAAAA1431950.065.17966563
GTAATCT1377650.065.0653337
TGAAAAA1431050.064.96583662
Produced by FastQC (version 0.11.2)