FastQCFastQC Report
Fri 31 Mar 2017
HGVKWBGX2_n01_sl40_grab_c11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGVKWBGX2_n01_sl40_grab_c11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11434467
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG6098065.333051378783113TruSeq Adapter, Index 11 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG483800.4231067351018635TruSeq Adapter, Index 11 (100% over 63bp)
CCCCCACCCACGAGGAGCATCGTGGAAAAAGAAGACGTTCCAACCACGTCTTCAAAGCAAGTGGATTGATGTGAT149280.13055265278215417No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT772650.066.974649
GCTTGAA774400.066.9262359
TGCTTGA774000.066.89403558
TATGCCG774300.066.8678246
CTTGAAA776650.066.8667360
CGTATGC776200.066.8454944
ATGCCGT774950.066.8286647
GTATGCC776050.066.7819745
TCTCGTA770200.066.781641
CCGTCTT774400.066.77482650
TGCCGTC775050.066.7672148
CATCTCG763250.066.7024139
ATCTCGT770500.066.6979840
TACATCT758750.066.541437
CTACATC755450.066.4402136
GCTACAT758850.066.3377235
CACGGCT774750.065.5813131
CTGCTTG800950.064.62343657
GAAAAAA819800.063.93979363
TTCTGCT811650.063.83212755
Produced by FastQC (version 0.11.2)