FastQCFastQC Report
Fri 31 Mar 2017
HGVKWBGX2_n01_sl40_grab_c10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGVKWBGX2_n01_sl40_grab_c10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10073141
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG9484889.416010358635901TruSeq Adapter, Index 10 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG876320.8699570471613571TruSeq Adapter, Index 10 (100% over 63bp)
CCCCCACCCACGAGGAGCATCGTGGAAAAAGAAGACGTTCCAACCACGTCTTCAAAGCAAGTGGATTGATGTGAT120950.1200717829721633No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATG104400.10364195239598056TruSeq Adapter, Index 10 (100% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCTTGA1260450.066.9314258
GCTTGAA1270650.066.8844559
CTTGAAA1279000.066.87694560
GCCGTCT1266750.066.8368849
TCTCGTA1284450.066.78474441
ATGCCGT1278850.066.7719947
TATGCCG1283100.066.7702646
CCGTCTT1278150.066.73804550
TGCCGTC1272300.066.7239548
CGTATGC1289350.066.72153544
GTATGCC1292300.066.7160645
ATCTCGT1291500.066.71300540
TTATCTC1289400.066.6387438
GCTTATC1300200.066.4702436
AGCTTAT1322700.066.3378335
TAGCTTA1324550.066.206134
ACTAGCT1335950.066.0543332
CACTAGC1344950.065.99161531
GTCACTA1360350.065.9114529
CTGCTTG1271200.065.80743457
Produced by FastQC (version 0.11.2)