FastQCFastQC Report
Fri 31 Mar 2017
HGVKWBGX2_n01_sl40_grab_c09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGVKWBGX2_n01_sl40_grab_c09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13857154
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG7123795.140875247543615TruSeq Adapter, Index 9 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG531270.3833904133561624TruSeq Adapter, Index 9 (100% over 63bp)
CCCCCACCCACGAGGAGCATCGTGGAAAAAGAAGACGTTCCAACCACGTCTTCAAAGCAAGTGGATTGATGTGAT192350.13880916673077315No Hit
CCCACGAGGAGCATCGTGGAAAAAGAAGACGTTCCAACCACGTCTTCAAAGCAAGTGGATTGATGTGATATCTCC149100.10759785162234611No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTGAA904650.067.24866559
CTTGAAA907800.067.2322660
GCCGTCT905850.067.2292649
TGCTTGA905000.067.1925758
ATGCCGT907250.067.1790147
TATGCCG907550.067.1622946
TGCCGTC906950.067.1510548
GTATGCC908600.067.1064945
CGTATGC909950.067.0984144
TCTCGTA891200.067.0719941
CCGTCTT907600.067.0545250
ATCTCGT890800.067.0118340
CACGATC906800.066.0679331
TCACGAT913150.066.0313930
CTGCTTG942100.064.5819857
GAAAAAA959700.064.3810463
TTCTGCT951200.064.027455
TCGTATG959750.063.91037843
TCTGAAC968950.063.82417318
TGAAAAA963250.063.8183762
Produced by FastQC (version 0.11.2)