Basic Statistics
Measure | Value |
---|---|
Filename | HGVKWBGX2_n01_sl40_grab_c08.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 20562189 |
Sequences flagged as poor quality | 0 |
Sequence length | 75 |
%GC | 48 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG | 1956866 | 9.516817494479794 | TruSeq Adapter, Index 8 (100% over 63bp) |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG | 176050 | 0.8561831622109883 | TruSeq Adapter, Index 8 (100% over 63bp) |
CCCCCACCCACGAGGAGCATCGTGGAAAAAGAAGACGTTCCAACCACGTCTTCAAAGCAAGTGGATTGATGTGAT | 27784 | 0.13512180050480035 | No Hit |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG | 23064 | 0.11216704602802746 | TruSeq Adapter, Index 8 (98% over 63bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TGCTTGA | 259455 | 0.0 | 67.13942 | 58 |
GCCGTCT | 260015 | 0.0 | 67.09869 | 49 |
GCTTGAA | 262035 | 0.0 | 67.07377 | 59 |
ATGCCGT | 263030 | 0.0 | 67.06518 | 47 |
AATCTCG | 262720 | 0.0 | 67.06063 | 39 |
TGCCGTC | 261515 | 0.0 | 67.031586 | 48 |
TCTCGTA | 261885 | 0.0 | 67.020515 | 41 |
CGTATGC | 265920 | 0.0 | 67.019844 | 44 |
TATGCCG | 264580 | 0.0 | 67.01831 | 46 |
GTATGCC | 266720 | 0.0 | 67.00493 | 45 |
CCGTCTT | 262765 | 0.0 | 67.00186 | 50 |
CTTGAAA | 264215 | 0.0 | 66.94701 | 60 |
ATCTCGT | 263175 | 0.0 | 66.94631 | 40 |
CACACTT | 277205 | 0.0 | 66.42613 | 31 |
GTCACAC | 281280 | 0.0 | 66.3846 | 29 |
TCACACT | 278895 | 0.0 | 66.36223 | 30 |
CTGCTTG | 262360 | 0.0 | 65.87437 | 57 |
TTCTGCT | 264965 | 0.0 | 65.583916 | 55 |
GAAAAAA | 279510 | 0.0 | 65.474464 | 63 |
TGAAAAA | 278000 | 0.0 | 65.43684 | 62 |