FastQCFastQC Report
Fri 31 Mar 2017
HGVKWBGX2_n01_sl40_grab_c08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGVKWBGX2_n01_sl40_grab_c08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20562189
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG19568669.516817494479794TruSeq Adapter, Index 8 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG1760500.8561831622109883TruSeq Adapter, Index 8 (100% over 63bp)
CCCCCACCCACGAGGAGCATCGTGGAAAAAGAAGACGTTCCAACCACGTCTTCAAAGCAAGTGGATTGATGTGAT277840.13512180050480035No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG230640.11216704602802746TruSeq Adapter, Index 8 (98% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCTTGA2594550.067.1394258
GCCGTCT2600150.067.0986949
GCTTGAA2620350.067.0737759
ATGCCGT2630300.067.0651847
AATCTCG2627200.067.0606339
TGCCGTC2615150.067.03158648
TCTCGTA2618850.067.02051541
CGTATGC2659200.067.01984444
TATGCCG2645800.067.0183146
GTATGCC2667200.067.0049345
CCGTCTT2627650.067.0018650
CTTGAAA2642150.066.9470160
ATCTCGT2631750.066.9463140
CACACTT2772050.066.4261331
GTCACAC2812800.066.384629
TCACACT2788950.066.3622330
CTGCTTG2623600.065.8743757
TTCTGCT2649650.065.58391655
GAAAAAA2795100.065.47446463
TGAAAAA2780000.065.4368462