FastQCFastQC Report
Fri 31 Mar 2017
HGVKWBGX2_n01_sl40_grab_c07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGVKWBGX2_n01_sl40_grab_c07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31162606
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG22945787.36324170064596TruSeq Adapter, Index 7 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG2201400.706423589862799TruSeq Adapter, Index 7 (100% over 63bp)
CCCCCACCCACGAGGAGCATCGTGGAAAAAGAAGACGTTCCAACCACGTCTTCAAAGCAAGTGGATTGATGTGAT398230.12779098128057712No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCTTGA2976350.067.1624858
CTTGAAA3006200.067.1259660
GCTTGAA2994650.067.1096859
GCCGTCT2988400.067.0935349
TCTCGTA3006450.067.07241
ATGCCGT3012600.067.0492647
TATGCCG3021800.067.0386446
TGCCGTC3003150.067.023948
CGTATGC3033700.067.01633544
GTATGCC3033350.067.0154645
CATCTCG2983500.067.00677539
ATCTCGT3017500.067.0055140
CCGTCTT2999450.066.9858450
TCATCTC2980450.066.8762838
ATCATCT2981950.066.81850437
GATCATC2991800.066.75075536
CACCAGA3122100.066.0042831
AGTCACC3153900.065.9220628
ACCAGAT3109950.065.8551332
CTGCTTG3052100.065.274857
Produced by FastQC (version 0.11.2)