FastQCFastQC Report
Fri 31 Mar 2017
HGVKWBGX2_n01_sl40_bzip1ab_i18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGVKWBGX2_n01_sl40_bzip1ab_i18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20206293
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA9050824.479208531718312TruSeq Adapter, Index 18 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAG792700.3923035264310975TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1171550.067.3104446
TATGCCG1166550.067.2921748
GTATGCC1170200.067.2919247
GCCGTCT1161900.067.2687151
TCCGCAC1158500.067.2664435
TGCCGTC1164550.067.2219550
ACGTCCG1167750.067.2105832
ATGCCGT1167850.067.2005449
CATCTCG1152250.067.17322541
CGTCCGC1164550.067.14921633
TCTCGTA1168700.067.1337943
CACGTCC1175900.067.0876831
GTCACGT1191350.067.0322729
ATCTCGT1165350.066.97169542
TCACGTC1184350.066.93136630
CACATCT1155750.066.9101239
CCGTCTT1171750.066.8744852
TGCTTGA1157700.066.6763660
GCTTGAA1163300.066.6692861
CTTGAAA1175800.066.3139862
Produced by FastQC (version 0.11.2)