FastQCFastQC Report
Fri 31 Mar 2017
HGVKWBGX2_n01_sl40_bzip1ab_i17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGVKWBGX2_n01_sl40_bzip1ab_i17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26029455
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG12225824.69691739608071RNA PCR Primer, Index 17 (100% over 43bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG933870.358774319324012RNA PCR Primer, Index 17 (100% over 43bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG1545100.067.4773346
CGTATGC1548850.067.41080544
GTATGCC1546500.067.4102445
GCCGTCT1546350.067.4083749
ATGCCGT1548500.067.3589547
TGCCGTC1547650.067.35268448
TCTCGTA1532950.067.2540541
ATCTCGT1530600.067.185540
CCGTCTT1552900.067.0973950
ACGTAGA1529100.067.0169732
GTCACGT1536650.067.01591529
TGCTTGA1557000.066.94466458
GCTTGAA1557450.066.94002559
CTTGAAA1568000.066.65874560
AGAGATC1532250.066.35921536
TCTGAAC1604250.066.3054518
CGTCTGA1608650.066.1726516
ACGTCTG1610950.066.1110415
CACACGT1617000.065.9944512
ACACGTC1618250.065.9941313
Produced by FastQC (version 0.11.2)