FastQCFastQC Report
Fri 31 Mar 2017
HGVKWBGX2_n01_sl40_bzip1ab_i16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGVKWBGX2_n01_sl40_bzip1ab_i16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24235548
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG10799774.456169095082975TruSeq Adapter, Index 16 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG927680.38277657266095244TruSeq Adapter, Index 16 (98% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCTCG1359500.067.43107639
GCCGTCT1387950.067.3558449
CGTATGC1403450.067.3353244
TATGCCG1399100.067.321446
TCTCGTA1378600.067.3027341
GTATGCC1402350.067.2972645
ATGCCGT1396900.067.265147
ATCTCGT1380200.067.24650640
TGCCGTC1394450.067.2335948
CCGTCCA1368450.067.1852534
CCGTCTT1392350.067.0858650
CCCGTCC1372800.067.05794533
TGCTTGA1392500.067.0420358
GCTTGAA1396500.066.99631559
CACCCGT1396950.066.9677331
TCACCCG1402000.066.95164530
ACCCGTC1390400.066.9362532
GTCACCC1413050.066.8052729
AGTCACC1428600.066.7877728
CTTGAAA1405450.066.7103160
Produced by FastQC (version 0.11.2)