FastQCFastQC Report
Fri 31 Mar 2017
HGVKWBGX2_n01_sl40_bzip1ab_i15.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGVKWBGX2_n01_sl40_bzip1ab_i15.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28018989
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA11230714.008249548190336TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCTTCTGCGTGAAAAAAAAAA587260.20959357241619245TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC1524250.067.3186647
AATCTCG1623850.067.2769441
TCTCGTA1580750.067.2734843
CGTATGC1518900.067.2522646
GCCGTCT1456450.067.2309951
TATGCCG1486000.067.23041548
ATGCCGT1487500.067.2209649
ATCTCGT1611550.067.2101842
TGCCGTC1477600.067.1558550
ATGTCAG1732200.067.032134
ACATGTC1743000.066.9548632
CCGTCTT1495700.066.9322852
GTCACAT1776500.066.8341729
GCTTGAA1393800.066.8077561
TGCTTGA1377750.066.5974260
CTTGAAA1438100.066.4205362
TCTGAAC1840350.066.253418
CGTCTGA1844650.066.1148516
ACGTCTG1844450.066.0986315
ACACGTC1853750.065.9528813
Produced by FastQC (version 0.11.2)