FastQCFastQC Report
Fri 31 Mar 2017
HGVKWBGX2_n01_sl40_bzip1ab_i14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGVKWBGX2_n01_sl40_bzip1ab_i14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences842857
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA12989715.411511086696795TruSeq Adapter, Index 14 (97% over 44bp)
GATCGGAATAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA179132.125271546656194TruSeq Adapter, Index 14 (97% over 41bp)
GATCTGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA162761.9310511747544366TruSeq Adapter, Index 14 (97% over 41bp)
GATCGTAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA97811.1604578238064107TruSeq Adapter, Index 14 (97% over 41bp)
GATAGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA80920.9600679593335524TruSeq Adapter, Index 14 (97% over 41bp)
TATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA71980.8540001447457873TruSeq Adapter, Index 14 (97% over 41bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG61980.7353560568400096No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAG48520.575661114518833TruSeq Adapter, Index 14 (97% over 44bp)
GATCGGAAGAGCACACGTCTTAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA28970.34371192266303774TruSeq Adapter, Index 14 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTACGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA25360.300881406929052TruSeq Adapter, Index 14 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCAGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA19570.2321864800316068TruSeq Adapter, Index 14 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCGTGAAAAAAAAAA18830.22340681752657923TruSeq Adapter, Index 14 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTAACAGTTCCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA16990.20157630535191617TruSeq Adapter, Index 14 (97% over 41bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA16390.1944576600775695TruSeq Adapter, Index 14 (97% over 41bp)
CATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA13330.15815256917840156TruSeq Adapter, Index 14 (97% over 41bp)
GATCAGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA11540.13691527744326737TruSeq Adapter, Index 14 (97% over 41bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAT11460.13596612474002115TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGCGA150.002354292269.0076558
AGTCTGC150.002355400568.99946656
CCGTATG150.002356509268.9912839
CCGTACC150.002356509268.9912833
GTTCCGT259400.067.63486535
AGTTCCG260950.067.6032634
TTCCGTA260300.067.59978536
CACAGTT274950.067.5946431
CGTATCT264800.067.5843539
TCCGTAT261950.067.5558837
TCACAGT277800.067.5344330
CGTCTGA290250.067.47002416
ACGTCTG290250.067.4343715
TCTGAAC290700.067.4249118
GCACACG295050.067.3738611
AGCACAC295800.067.34296410
CACACGT296750.067.30173512
ACACGTC296400.067.2997413
ACTCCAG293650.067.2956923
GAGCACA267750.067.286679
Produced by FastQC (version 0.11.2)