FastQCFastQC Report
Fri 31 Mar 2017
HGVKWBGX2_n01_sl40_bzip1ab_i13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGVKWBGX2_n01_sl40_bzip1ab_i13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15067892
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA7686035.1009324993834575TruSeq Adapter, Index 13 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAG514630.34154080743344856TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACAGTC993350.067.13988531
AGTCAAC985500.067.0756334
CGTATGC992350.067.0482946
GCCGTCT984050.067.0199851
GTATGCC991500.067.0139747
TCACAGT1001550.067.0097530
ATGCCGT989200.067.00957549
AATCTCG973650.067.0021941
TGCCGTC986950.066.9624350
TCTCGTA980900.066.9470443
TATGCCG990300.066.9442648
ACAGTCA996800.066.9319532
ATCTCGT977750.066.904742
CCGTCTT989650.066.79391552
AACAATC980150.066.7626438
GCTTGAA982500.066.6651561
TGCTTGA978550.066.6605460
CTTGAAA991650.066.4893862
TCTGAAC1050500.066.4781618
CGTCTGA1052650.066.358116
Produced by FastQC (version 0.11.2)