FastQCFastQC Report
Fri 31 Mar 2017
HGVKWBGX2_n01_sl40_bzip1ab_c06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGVKWBGX2_n01_sl40_bzip1ab_c06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24517862
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG590728024.093781097226177TruSeq Adapter, Index 6 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG4925462.008927205806118TruSeq Adapter, Index 6 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATG495760.2022036016027825TruSeq Adapter, Index 6 (100% over 63bp)
CATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG355370.14494330704691952TruSeq Adapter, Index 6 (98% over 63bp)
TATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG330150.13465692889534983TruSeq Adapter, Index 6 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG295990.12072422954334273TruSeq Adapter, Index 6 (98% over 63bp)
GATAGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG277330.11311345173571823TruSeq Adapter, Index 6 (98% over 63bp)
CCCCCACCCACGAGGAGCATCGTGGAAAAAGAAGACGTTCCAACCACGTCTTCAAAGCAAGTGGATTGATGTGAT264850.108023285227725No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTGAA7389250.067.5522559
TGCTTGA7381800.067.550458
GCCGTCT7382300.067.5320149
TATGCCG7397850.067.53065546
GTATGCC7400350.067.5236945
CGTATGC7411550.067.5215344
TCTCGTA7368950.067.5195641
TGCCGTC7387800.067.51887548
ATGCCGT7396000.067.51845647
CTTGAAA7412350.067.5138160
CCGTCTT7383350.067.4993850
ATCTCGT7374200.067.4918440
CAATATC7272100.067.3920936
AATATCT7327100.067.38541437
GCCAATA7279000.067.34125534
CCAATAT7283450.067.338935
CGCCAAT7279150.067.3375933
CACGCCA7337450.067.2592231
TCACGCC7338900.067.2061130
TATCTCG7367850.067.1363339
Produced by FastQC (version 0.11.2)