FastQCFastQC Report
Fri 31 Mar 2017
HGVKWBGX2_n01_sl40_bzip1ab_c05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGVKWBGX2_n01_sl40_bzip1ab_c05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25081790
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG711563428.36972161875209TruSeq Adapter, Index 5 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG6256212.4943235710050997TruSeq Adapter, Index 5 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATG686550.2737244829814778TruSeq Adapter, Index 5 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG472210.18826806220768136TruSeq Adapter, Index 5 (98% over 63bp)
TATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG464160.1850585624072285TruSeq Adapter, Index 5 (98% over 63bp)
CATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG456510.18200854085772986TruSeq Adapter, Index 5 (98% over 63bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG394700.15736516412903545TruSeq Adapter, Index 5 (98% over 63bp)
GATAGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG310260.1236993053526084TruSeq Adapter, Index 5 (98% over 63bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA8993750.067.8064441
ATCTCGT8985050.067.78950540
AGTGATC8937450.067.7354636
TGCTTGA9074600.067.7039158
GCCGTCT9098950.067.6979949
GCTTGAA9117650.067.68854559
CGTATGC9176900.067.6866844
TATGCCG9157800.067.68481446
GTATGCC9185250.067.68207645
ATGCCGT9148350.067.6756947
TGCCGTC9117400.067.6740848
CCGTCTT9121750.067.6676950
CTTGAAA9161600.067.6344960
CAGTGAT9118550.067.5451335
CACAGTG9177800.067.4884333
ACACAGT9220150.067.48624432
CTCGTAT9059050.067.4460342
GTCACAC9308800.067.442229
TCGTATG9215750.067.3568443
TTCTGCT9089600.067.3506855
Produced by FastQC (version 0.11.2)