FastQCFastQC Report
Fri 31 Mar 2017
HGVKWBGX2_n01_sl40_bzip1ab_c04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGVKWBGX2_n01_sl40_bzip1ab_c04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27108424
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG19931557.352529973708542TruSeq Adapter, Index 4 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG1681070.6201282671393955TruSeq Adapter, Index 4 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG428780.15817223457918467TruSeq Adapter, Index 4 (98% over 63bp)
CCCCCACCCACGAGGAGCATCGTGGAAAAAGAAGACGTTCCAACCACGTCTTCAAAGCAAGTGGATTGATGTGAT398610.14704285280472223No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT2587350.067.4435549
CGTATGC2599800.067.417644
TATGCCG2594750.067.4069246
GTATGCC2596850.067.39973445
TGCCGTC2591250.067.3889848
TCTCGTA2509100.067.3778941
ATGCCGT2595600.067.3672847
AATCTCG2497700.067.35847539
ATCTCGT2508950.067.321840
CCGTCTT2593300.067.2820350
TGCTTGA2599000.067.235358
GCTTGAA2601850.067.221459
ACCAATC2457100.067.211536
ACTGACC2545350.067.0924232
CTTGAAA2615350.067.0461560
AGTCACT2630150.066.7324828
TCTGAAC2716300.065.76337418
CGTCTGA2724000.065.58729616
ACGTCTG2729350.065.4462815
CTGCTTG2669800.065.39371557