Basic Statistics
Measure | Value |
---|---|
Filename | HGVKWBGX2_n01_sl40_bzip1ab_c04.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 27108424 |
Sequences flagged as poor quality | 0 |
Sequence length | 75 |
%GC | 48 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG | 1993155 | 7.352529973708542 | TruSeq Adapter, Index 4 (100% over 63bp) |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG | 168107 | 0.6201282671393955 | TruSeq Adapter, Index 4 (100% over 63bp) |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG | 42878 | 0.15817223457918467 | TruSeq Adapter, Index 4 (98% over 63bp) |
CCCCCACCCACGAGGAGCATCGTGGAAAAAGAAGACGTTCCAACCACGTCTTCAAAGCAAGTGGATTGATGTGAT | 39861 | 0.14704285280472223 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
GCCGTCT | 258735 | 0.0 | 67.44355 | 49 |
CGTATGC | 259980 | 0.0 | 67.4176 | 44 |
TATGCCG | 259475 | 0.0 | 67.40692 | 46 |
GTATGCC | 259685 | 0.0 | 67.399734 | 45 |
TGCCGTC | 259125 | 0.0 | 67.38898 | 48 |
TCTCGTA | 250910 | 0.0 | 67.37789 | 41 |
ATGCCGT | 259560 | 0.0 | 67.36728 | 47 |
AATCTCG | 249770 | 0.0 | 67.358475 | 39 |
ATCTCGT | 250895 | 0.0 | 67.3218 | 40 |
CCGTCTT | 259330 | 0.0 | 67.28203 | 50 |
TGCTTGA | 259900 | 0.0 | 67.2353 | 58 |
GCTTGAA | 260185 | 0.0 | 67.2214 | 59 |
ACCAATC | 245710 | 0.0 | 67.2115 | 36 |
ACTGACC | 254535 | 0.0 | 67.09242 | 32 |
CTTGAAA | 261535 | 0.0 | 67.04615 | 60 |
AGTCACT | 263015 | 0.0 | 66.73248 | 28 |
TCTGAAC | 271630 | 0.0 | 65.763374 | 18 |
CGTCTGA | 272400 | 0.0 | 65.587296 | 16 |
ACGTCTG | 272935 | 0.0 | 65.44628 | 15 |
CTGCTTG | 266980 | 0.0 | 65.393715 | 57 |