FastQCFastQC Report
Fri 31 Mar 2017
HGVKWBGX2_n01_sl40_bzip1ab_c03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGVKWBGX2_n01_sl40_bzip1ab_c03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17840513
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG9983935.596212395910364TruSeq Adapter, Index 3 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG884700.49589381202210947TruSeq Adapter, Index 3 (100% over 63bp)
CCCCCACCCACGAGGAGCATCGTGGAAAAAGAAGACGTTCCAACCACGTCTTCAAAGCAAGTGGATTGATGTGAT253440.1420586952852757No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA1290000.067.5796741
CGTATGC1305750.067.5601644
GCCGTCT1295800.067.53448549
CATCTCG1279100.067.51852439
TATGCCG1303450.067.4901346
GTATGCC1306350.067.48743445
ATCTCGT1291200.067.48347540
TGCCGTC1300300.067.45653548
ATGCCGT1304100.067.44118547
GCATCTC1274350.067.4406938
GCTTGAA1304000.067.37057559
TGCTTGA1299700.067.3705158
CCGTCTT1301500.067.3292150
TAGGCAT1283450.067.2773235
CTTGAAA1312700.067.1771460
CACTTAG1315850.066.8249231
AGTCACT1338700.066.66003428
TCACTTA1330500.066.5678830
ACTTAGG1312600.066.53342432
TCTGAAC1380500.065.8412918
Produced by FastQC (version 0.11.2)