FastQCFastQC Report
Fri 31 Mar 2017
HGVKWBGX2_n01_sl40_bzip1ab_c02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGVKWBGX2_n01_sl40_bzip1ab_c02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26945660
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG18611886.9071902488192904TruSeq Adapter, Index 2 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG1711650.6352228893261475TruSeq Adapter, Index 2 (100% over 63bp)
CCCCCACCCACGAGGAGCATCGTGGAAAAAGAAGACGTTCCAACCACGTCTTCAAAGCAAGTGGATTGATGTGAT412280.15300423147920666No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG2407350.067.4777146
GCCGTCT2389100.067.4749
CGTATGC2413000.067.4673444
GTATGCC2416050.067.44021645
ATGCCGT2404700.067.4190847
TCTCGTA2396400.067.41437541
TGCCGTC2396700.067.3878548
ATCTCGT2403800.067.28689640
CCGTCTT2401150.067.26214650
TGCTTGA2395750.067.21622558
GCTTGAA2406350.067.2147259
CCGATGT2400400.067.1253233
GATGTAT2405850.067.0984535
CTTGAAA2422600.067.04932460
ACCGATG2420200.066.9936932
AGTCACC2472950.066.78474428
GTCACCG2449100.066.7843429
TCACCGA2450850.066.7742430
TCTGAAC2565200.065.8062418
CGTCTGA2576650.065.576516
Produced by FastQC (version 0.11.2)