FastQCFastQC Report
Fri 31 Mar 2017
HGVKWBGX2_n01_sl40_bzip1ab_c01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGVKWBGX2_n01_sl40_bzip1ab_c01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19726837
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG17222548.7305126513693TruSeq Adapter, Index 1 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG1335090.6767886813278784TruSeq Adapter, Index 1 (100% over 63bp)
CCCCCACCCACGAGGAGCATCGTGGAAAAAGAAGACGTTCCAACCACGTCTTCAAAGCAAGTGGATTGATGTGAT272240.13800489150896314No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT2239050.067.4813549
GTATGCC2245200.067.4665545
CGTATGC2248650.067.4661744
TATGCCG2243700.067.4600646
ATGCCGT2243600.067.44649547
TCTCGTA2201050.067.4412741
TGCCGTC2242000.067.4276148
ATCTCGT2197300.067.3875240
CACGATC2147200.067.3609536
CCGTCTT2241450.067.3477150
TGCTTGA2245950.067.339458
GCTTGAA2246250.067.3259759
TCACGAT2180600.067.29285435
CTTGAAA2258850.067.17004460
ATCACGA2194000.067.158134
GTCACAT2257200.066.98917429
TCTGAAC2330250.066.3050718
CGTCTGA2336450.066.1179516
ACGTCTG2339850.066.0182615
TCGTATG2314400.065.7982743
Produced by FastQC (version 0.11.2)