FastQCFastQC Report
Fri 25 Aug 2017
HGVK2BGX3_n01_nip_36hr_cpa_set2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGVK2BGX3_n01_nip_36hr_cpa_set2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32650949
Sequences flagged as poor quality0
Sequence length75
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGGTTCCTCCCGCTCCGGAACCCCCGCGGGGTTGGAC2718820.8326924892749672No Hit
GTCCAGAGTCGCCGCCGCCGCCGGCCCCCCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGGTTCCTC2572990.7880291626439403No Hit
CCCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGGTTCCTCCCGCTCCGGAACCCCCGCGGGGTTGGA2079320.6368329447330918No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGT1956760.5992965166188585No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCC1504260.4607094268531062No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCG1498760.45902494288910256No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCAT1361330.41693428267582666No Hit
GGCCCGGCTCGCGTCCAGAGTCGCCGCCGCCGCCGGCCCCCCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCC1184850.3628837863181251No Hit
GTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGC1083490.3318402782105966No Hit
CCCCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGGTTCCTCCCGCTCCGGAACCCCCGCGGGGTTGG980190.30020260666849224No Hit
GCCCCCCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGGTTCCTCCCGCTCCGGAACCCCCGCGGGGT941590.2883805919393032No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATA914890.2802031879685947No Hit
GTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGCGCGCGGAGGGGAGGCGGGGGACGGGCCCCCGCCACGCA908650.2782920643439797No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACC833220.2551901324521992No Hit
GTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGCGGGCCCGGGCGGAAAGGACGGGGGG739120.22637014317715543No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACCC732140.224232379891929No Hit
GGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGCGGGCCCGGGCGGAA697250.21354662616391334No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACCCG691020.2116385652374147No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTC628030.1923466298023987No Hit
GCGGCGACGGGTATCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAACACTCGGGG594690.18213559428242038No Hit
CGGGTATCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAACACTCGGGGTGAGGTG578520.17718321142824978No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTT566450.1734865348017909No Hit
GCCCGGCTCGCGTCCAGAGTCGCCGCCGCCGCCGGCCCCCCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCC563800.1726749198009528No Hit
GCGACGGGTATCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAACACTCGGGGTGA553510.16952340343920785No Hit
GGGCCCGGCTCGCGTCCAGAGTCGCCGCCGCCGCCGGCCCCCCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGC551090.16878223049504626No Hit
CCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCG549730.16836570355121988No Hit
GGTGGAAGTGCGCCCGGCGGCGGCCGGTCGCCGGCCGGGGGGCGGTCCCCCGCCGACCCCACCCCCGGCCCCGCC547300.16762146790894195No Hit
GCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGCGCGCGGAGGGGAGGCGGGGGACGGGCCCCCGCCACG509810.15613941267067No Hit
CTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAACACTCGGGGTGAGGTGGTTCGGC499310.15292357964848127No Hit
GGCGCCGCGCGGAAAACCGCGGCCCGGGGGGCGGACCCGGCGGGGGAACACCGACGCGGAGGTTCCCCCCACACG489240.14983944264529647No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGT485180.14859598721005016No Hit
GACGGGTATCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAACACTCGGGGTGAGG478210.1464612866229401No Hit
GGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGG474870.14543834545207246No Hit
TGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGG469210.14370485831820692No Hit
CCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGGTTCCTCCCGCTCCGGAACCCCCGCGGGGTTGGACC463650.1420019981655051No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA444440.1361185550839579TruSeq Adapter, Index 25 (97% over 44bp)
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCAAACTTAGTGCGGACACC440800.13500373296959914No Hit
GACGGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCAC437080.13386440927030943No Hit
GGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGCGGGCCCGGGCGGAAA427650.1309762849465723No Hit
GCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGG423890.1298247104548171No Hit
CCCGGCTCGCGTCCAGAGTCGCCGCCGCCGCCGGCCCCCCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCG419150.12837299154765763No Hit
GGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAG408530.12512040614807246No Hit
GGCCCCCCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGGTTCCTCCCGCTCCGGAACCCCCGCGGGG394850.12093063512487799No Hit
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTT386780.11845903774496723No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCAAACTTAGTGCGGACACCC381390.1168082434602437No Hit
CCAGAGTCGCCGCCGCCGCCGGCCCCCCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGGTTCCTCCC377410.11558928960992833No Hit
ACGGGAAGGGCCCGGCTCGCGTCCAGAGTCGCCGCCGCCGCCGGCCCCCCCGAGTGTCCGGGCCCCCCGCCCCAC371760.11385886517417916No Hit
GTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGT365640.1119844939269606No Hit
TCCAGAGTCGCCGCCGCCGCCGGCCCCCCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGGTTCCTCC361030.11057259009531392No Hit
GTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGCGCGCGGAGGGGAGGCGGGGGACGGGCCCCCGCCACGCC354910.10869821884809536No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCAAACTTAGTGCGGACACCCG352520.10796623399828287No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACCCGATC345460.1058039691281255No Hit
GGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGCGGGCCCGGGCGGAAAGGACGGGGGGTC344110.10539050488241551No Hit
CCCCCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGGTTCCTCCCGCTCCGGAACCCCCGCGGGGTTG343600.10523430727848063No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCTTGAGAGCTTGTTTGGAGGTTCTAGC335940.10288828052134105No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG65050.047.78536648
TCGTATG66450.047.14188445
CGTATGC71350.043.8076946
TATCTCG71950.043.2026241
GTATGCC76150.040.95578847
ATGCCGT77600.040.19056749
GTTGGAC621400.036.20998469
TGCCGTC87650.036.0545750
TCTCGTA86650.036.03275343
GCCGTCT87800.035.9536851
GCCACGC272200.033.85357368
ATCTCGT94200.033.51096742
CCACGCA199200.033.42617869
ATATATC94200.033.25449838
ATATCTC98300.031.83243640
TATATCT99800.031.49226839
GATATAT101900.030.84311937
GTCGGCT67750.030.7069071
CCGTCTT106250.029.48322752
TAGGCGC64050.029.41008410
Produced by FastQC (version 0.11.2)