FastQCFastQC Report
Fri 25 Aug 2017
HGVK2BGX3_n01_nip_36hr_con_set2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGVK2BGX3_n01_nip_36hr_con_set2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35665407
Sequences flagged as poor quality0
Sequence length75
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGGTTCCTCCCGCTCCGGAACCCCCGCGGGGTTGGAC1589100.4455577921766041No Hit
CCCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGGTTCCTCCCGCTCCGGAACCCCCGCGGGGTTGGA1106250.31017450606970504No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGT1036720.2906794250238053No Hit
GTCCAGAGTCGCCGCCGCCGCCGGCCCCCCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGGTTCCTC956770.2682627454664964No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCG804060.22544534540149788No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCC743930.2085858714580209No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCAT613820.17210514378820913No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG597080.1674115200760221No Hit
GTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGC561840.15753079727927963No Hit
GCCCCCCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGGTTCCTCCCGCTCCGGAACCCCCGCGGGGT507340.14224988375991338No Hit
GGCCCGGCTCGCGTCCAGAGTCGCCGCCGCCGCCGGCCCCCCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCC504110.1413442442981234No Hit
CCCCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGGTTCCTCCCGCTCCGGAACCCCCGCGGGGTTGG486590.13643192127318216No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATA422980.1185967119343402No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACC383550.10754118129087942No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG46750.037.78361548
GTTGGAC393450.034.22352669
TCGTATG54100.032.5865845
CGTATGC55700.031.77435546
TATCTCG60300.029.52236741
ATGCCGT69950.025.59740849
GTATGCC70800.025.48490347
GGTTGGA573950.023.65902168
ACCGCTA34750.023.6295242
TGCCGTC85100.021.24310950
AACCCGG136250.020.73796830
TCTCGTA85650.020.70398343
TCGCGGT32150.020.6040732
GCCGTCT88700.020.53648251
CGGGGTT671950.020.52661365
GCGAAAC140750.020.07480226
ATCTCGT91900.019.59628542
GCCACGC131450.019.26421268
CCGCTAA45850.019.1130073
CGAAACC150750.018.9491127
Produced by FastQC (version 0.11.2)