FastQCFastQC Report
Fri 25 Aug 2017
HGVK2BGX3_n01_nip_0hr_con_set2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGVK2BGX3_n01_nip_0hr_con_set2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38107400
Sequences flagged as poor quality0
Sequence length75
%GC69

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGAGTCGCCGCCGCCGCCGGCCCCCCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGGTTCCTC7066211.8542881435101843No Hit
CCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGGTTCCTCCCGCTCCGGAACCCCCGCGGGGTTGGAC4892461.2838608774148852No Hit
GGCCCGGCTCGCGTCCAGAGTCGCCGCCGCCGCCGGCCCCCCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCC3395660.8910762738995576No Hit
CCCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGGTTCCTCCCGCTCCGGAACCCCCGCGGGGTTGGA2978730.781667077785417No Hit
CGGGTATCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAACACTCGGGGTGAGGTG2670670.7008271359368522No Hit
GCGGCGACGGGTATCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAACACTCGGGG2482440.6514325301647449No Hit
GCGACGGGTATCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAACACTCGGGGTGA2291560.6013425214000431No Hit
GTCCCGGAGCCGGTCGCGGCGCACCGCCACGGTGGAAGTGCGCCCGGCGGCGGCCGGTCGCCGGCCGGGGGGCGG2250690.5906175703406688No Hit
GGTGGAAGTGCGCCCGGCGGCGGCCGGTCGCCGGCCGGGGGGCGGTCCCCCGCCGACCCCACCCCCGGCCCCGCC2214660.5811627138036182No Hit
CTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAACACTCGGGGTGAGGTGGTTCGGC2209260.5797456661960669No Hit
GCCCGGCTCGCGTCCAGAGTCGCCGCCGCCGCCGGCCCCCCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCC1926660.5055868414008828No Hit
CCCCACCGGGCCTTCCCGGCCGTCCCGGAGCCGGTCGCGGCGCACCGCCACGGTGGAAGTGCGCCCGGCGGCGGC1796030.4713074101093226No Hit
CCCGGCTCGCGTCCAGAGTCGCCGCCGCCGCCGGCCCCCCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCG1687140.4427329075192745No Hit
GACGGGTATCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAACACTCGGGGTGAGG1655950.4345481455045477No Hit
GTGGAAGTGCGCCCGGCGGCGGCCGGTCGCCGGCCGGGGGGCGGTCCCCCGCCGACCCCACCCCCGGCCCCGCCC1479870.3883418968494308No Hit
GCCCCCCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGGTTCCTCCCGCTCCGGAACCCCCGCGGGGT1418610.3722662789904323No Hit
GGGTATCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAACACTCGGGGTGAGGTGG1406590.3691120359825126No Hit
GGGCCCGGCTCGCGTCCAGAGTCGCCGCCGCCGCCGGCCCCCCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGC1347050.3534877740281415No Hit
CCCGGAGCCGGTCGCGGCGCACCGCCACGGTGGAAGTGCGCCCGGCGGCGGCCGGTCGCCGGCCGGGGGGCGGTC1340130.3516718537606869No Hit
CCCCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGGTTCCTCCCGCTCCGGAACCCCCGCGGGGTTGG1325250.34776710035321223No Hit
CTTCCCCACCGGGCCTTCCCGGCCGTCCCGGAGCCGGTCGCGGCGCACCGCCACGGTGGAAGTGCGCCCGGCGGC1316770.3455418107769095No Hit
GCCACCTTCCCCACCGGGCCTTCCCGGCCGTCCCGGAGCCGGTCGCGGCGCACCGCCACGGTGGAAGTGCGCCCG1313970.3448070453507718No Hit
TCCAGAGTCGCCGCCGCCGCCGGCCCCCCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGGTTCCTCC1280600.33605021596855206No Hit
TCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAACACTCGGGGTGAGGTGGTTCGG1219810.3200979337346552No Hit
CCAGAGTCGCCGCCGCCGCCGGCCCCCCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGGTTCCTCCC1193260.313130782997528No Hit
AGCGCGGCGACGGGTATCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAACACTCG1111050.2915575452536778No Hit
GCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAACACTCGGGGTGAGGTGGTTCGGCGCG1108440.29087263891002796No Hit
GGCGACGGGTATCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAACACTCGGGGTG1023890.2686853472029055No Hit
ACGGGAAGGGCCCGGCTCGCGTCCAGAGTCGCCGCCGCCGCCGGCCCCCCCGAGTGTCCGGGCCCCCCGCCCCAC994850.261064780068963No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGT991100.26008071923038567No Hit
GGCGCCGCGCGGAAAACCGCGGCCCGGGGGGCGGACCCGGCGGGGGAACACCGACGCGGAGGTTCCCCCCACACG921230.24174569768601376No Hit
CGCGGCGACGGGTATCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAACACTCGGG910540.23894046825550944No Hit
CTCGGCTTCGTCGGGAGACGCGTGACCGACGGTCCCCCCGGGACCCGACGGCGCGACGACGCCCGGGGCGCACTG898790.2358570776279673No Hit
ATCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAACACTCGGGGTGAGGTGGTTCG852660.22375181723234858No Hit
CACGGGAAGGGCCCGGCTCGCGTCCAGAGTCGCCGCCGCCGCCGGCCCCCCCGAGTGTCCGGGCCCCCCGCCCCA839300.22024593648477722No Hit
GGGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGCGGGCCCGGGCGGAA825540.21663508924775768No Hit
CCACGGGAAGGGCCCGGCTCGCGTCCAGAGTCGCCGCCGCCGCCGGCCCCCCCGAGTGTCCGGGCCCCCCGCCCC818640.21482441730477547No Hit
CCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGGTTCCTCCCGCTCCGGAACCCCCGCGGGGTTGGACC808520.21216876512173488No Hit
GCGCCGCGCGGAAAACCGCGGCCCGGGGGGCGGACCCGGCGGGGGAACACCGACGCGGAGGTTCCCCCCACACGC803270.21079107994772667No Hit
CGGCGACGGGTATCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAACACTCGGGGT754070.19788020174559273No Hit
CCCCGAGCCACCTTCCCCACCGGGCCTTCCCGGCCGTCCCGGAGCCGGTCGCGGCGCACCGCCACGGTGGAAGTG752110.19736586594729633No Hit
AGGCGATCCACGGGAAGGGCCCGGCTCGCGTCCAGAGTCGCCGCCGCCGCCGGCCCCCCCGAGTGTCCGGGCCCC736040.19314883723371312No Hit
CCGGAGCCGGTCGCGGCGCACCGCCACGGTGGAAGTGCGCCCGGCGGCGGCCGGTCGCCGGCCGGGGGGCGGTCC718060.18843059353301458No Hit
GTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGCGGGCCCGGGCGGAAAGGACGGGGGG699220.1834866718800023No Hit
CGACGGGTATCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAACACTCGGGGTGAG698770.18336858457937305No Hit
GCGCGGCGACGGGTATCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAACACTCGG694860.18234253714501644No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCAT661730.17364868765646568No Hit
CCACGGTGGAAGTGCGCCCGGCGGCGGCCGGTCGCCGGCCGGGGGGCGGTCCCCCGCCGACCCCACCCCCGGCCC661710.17364343933199328No Hit
CCGGCTCGCGTCCAGAGTCGCCGCCGCCGCCGGCCCCCCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGC650360.17066501519389934No Hit
GTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGCGCGCGGAGGGGAGGCGGGGGACGGGCCCCCGCCACGCA642980.16872838346357924No Hit
CACGGTGGAAGTGCGCCCGGCGGCGGCCGGTCGCCGGCCGGGGGGCGGTCCCCCGCCGACCCCACCCCCGGCCCC630400.165427187370432No Hit
GGGAAGGGCCCGGCTCGCGTCCAGAGTCGCCGCCGCCGCCGGCCCCCCCGAGTGTCCGGGCCCCCCGCCCCACCG629820.16527498596073203No Hit
GCTCGGCTTCGTCGGGAGACGCGTGACCGACGGTCCCCCCGGGACCCGACGGCGCGACGACGCCCGGGGCGCACT628330.16488398578753732No Hit
CCCACCGGGCCTTCCCGGCCGTCCCGGAGCCGGTCGCGGCGCACCGCCACGGTGGAAGTGCGCCCGGCGGCGGCC627670.16471079107994774No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCC626650.16444312653185472No Hit
GGAGGGGCGGCCCGGCCCCCACCCCCACGCCCGCCCGGGAGGCGGACGGGGGGAGAGGGAGAGCGCGGCGACGGG601050.15772527120716712No Hit
CCACCTTCCCCACCGGGCCTTCCCGGCCGTCCCGGAGCCGGTCGCGGCGCACCGCCACGGTGGAAGTGCGCCCGG598570.157074478972588No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCG596060.15641581425130027No Hit
CTCGCGTCCAGAGTCGCCGCCGCCGCCGGCCCCCCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGGT593200.15566530385174535No Hit
GCGTCCAGAGTCGCCGCCGCCGCCGGCCCCCCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGGTTCC582100.15275248376955655No Hit
CGGGAAGGGCCCGGCTCGCGTCCAGAGTCGCCGCCGCCGCCGGCCCCCCCGAGTGTCCGGGCCCCCCGCCCCACC580820.1524165910033222No Hit
GGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAACACTCGGGGTGAGGTGGTTCGGCGC561570.14736507869862545No Hit
ACGGGTATCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAACACTCGGGGTGAGGT556720.1460923600140655No Hit
GGGAGAGCGGTCGCGCCGTGGGAGGGGCGGCCCGGCCCCCACCCCCACGCCCGCCCGGGAGGCGGACGGGGGGAG529590.13897300786723837No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATA524420.13761631599111984No Hit
GCCGGCCCCCCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGGTTCCTCCCGCTCCGGAACCCCCGCG506030.13279048163873683No Hit
GGGAAGAGAGGTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGCGGGCCCGGGCGGAAA502280.13180642080015953No Hit
GGCGAAAGCTGCGGCCGGAGGGCTGTAACACTCGGGGTGAGGTGGTTCGGCGCGCCCTGAGACGCGCCGCCCCCC495070.12991439982785496No Hit
GGCCCCCCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGGTTCCTCCCGCTCCGGAACCCCCGCGGGG487640.12796464728635382No Hit
GGGAGGGGCGGCCCGGCCCCCACCCCCACGCCCGCCCGGGAGGCGGACGGGGGGAGAGGGAGAGCGCGGCGACGG486190.12758414376210395No Hit
GGTATCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAACACTCGGGGTGAGGTGGT484020.12701470055684722No Hit
GCCGCGCGGAAAACCGCGGCCCGGGGGGCGGACCCGGCGGGGGAACACCGACGCGGAGGTTCCCCCCACACGCGC480920.12620121026362333No Hit
CCTTCCCCACCGGGCCTTCCCGGCCGTCCCGGAGCCGGTCGCGGCGCACCGCCACGGTGGAAGTGCGCCCGGCGG478210.1254900622976115No Hit
CCCGGCCGTCCCGGAGCCGGTCGCGGCGCACCGCCACGGTGGAAGTGCGCCCGGCGGCGGCCGGTCGCCGGCCGG475060.12466345119320657No Hit
GTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGC474620.12454798805481349No Hit
GCCACGGTGGAAGTGCGCCCGGCGGCGGCCGGTCGCCGGCCGGGGGGCGGTCCCCCGCCGACCCCACCCCCGGCC473150.12416223620609121No Hit
GTCGCCGCCGCCGCCGGCCCCCCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGGTTCCTCCCGCTCC468730.1230023564976881No Hit
GTCGGCTGCTAGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAAAACCGCGGCCCGGGGGGCGGACCCGGCGGGGGA466060.12230170518062108No Hit
TCCCCACCGGGCCTTCCCGGCCGTCCCGGAGCCGGTCGCGGCGCACCGCCACGGTGGAAGTGCGCCCGGCGGCGG464840.12198155738780395No Hit
CCGGGCCTTCCCGGCCGTCCCGGAGCCGGTCGCGGCGCACCGCCACGGTGGAAGTGCGCCCGGCGGCGGCCGGTC462660.12140949002031101No Hit
CCCCCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGGTTCCTCCCGCTCCGGAACCCCCGCGGGGTTG437720.11486482940321302No Hit
CACCGGGCCTTCCCGGCCGTCCCGGAGCCGGTCGCGGCGCACCGCCACGGTGGAAGTGCGCCCGGCGGCGGCCGG436790.11462078231524585No Hit
GTCGCGGCGCACCGCCACGGTGGAAGTGCGCCCGGCGGCGGCCGGTCGCCGGCCGGGGGGCGGTCCCCCGCCGAC432020.11336905692857556No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACC426170.11183392202039498No Hit
CACCTTCCCCACCGGGCCTTCCCGGCCGTCCCGGAGCCGGTCGCGGCGCACCGCCACGGTGGAAGTGCGCCCGGC421220.11053496171347296No Hit
GCCGGTCGCGGCGCACCGCCACGGTGGAAGTGCGCCCGGCGGCGGCCGGTCGCCGGCCGGGGGGCGGTCCCCCGC404480.10614211413006397No Hit
GTCGCGCCGTGGGAGGGGCGGCCCGGCCCCCACCCCCACGCCCGCCCGGGAGGCGGACGGGGGGAGAGGGAGAGC395840.10387483795798191No Hit
GGCTCGCGTCCAGAGTCGCCGCCGCCGCCGGCCCCCCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTG390950.10259162262447714No Hit
GTGGCGACGGGTATCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAACACTCGGGG383850.10072846743677082No Hit
GTCGGGAGACGCGTGACCGACGGTCCCCCCGGGACCCGACGGCGCGACGACGCCCGGGGCGCACTGGGGACAGTC383250.10057101770259845No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGGA81300.043.157031
GTCGGCT98350.040.7968331
GTTGGAC1048100.039.4904169
TATGCCG25450.037.0076946
TCGGCTG115150.034.365612
CGTATGC28100.032.90376744
GGAGTCG101500.032.6651155
CGGAGTC102550.032.36434
TAGGCGC127050.031.66227710
CTAGGCG129250.031.043399
TCGTATG30700.030.34180543
GCCACGC218700.030.1462768
CCGGAGT119150.028.9556143
TCGCGGT30650.028.9285852
CCACGCA164600.028.58947669
GGTTGGA1457950.028.42933568
GCTAGGC145200.027.705118
GTATGCC34900.026.78927845
TGCTAGG152600.026.7231737
GCTGCTA150700.026.6252235
Produced by FastQC (version 0.11.2)