FastQCFastQC Report
Fri 25 Aug 2017
HGVK2BGX3_n01_nil_12hr_cpa_set2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGVK2BGX3_n01_nil_12hr_cpa_set2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23843088
Sequences flagged as poor quality0
Sequence length75
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGGTTCCTCCCGCTCCGGAACCCCCGCGGGGTTGGAC1426180.5981523869726941No Hit
GTCCAGAGTCGCCGCCGCCGCCGGCCCCCCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGGTTCCTC1307480.5483685670245397No Hit
CCCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGGTTCCTCCCGCTCCGGAACCCCCGCGGGGTTGGA854050.3581960524576347No Hit
GGCCCGGCTCGCGTCCAGAGTCGCCGCCGCCGCCGGCCCCCCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCC702690.29471434237041777No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGT564620.23680657471884511No Hit
GCCCCCCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGGTTCCTCCCGCTCCGGAACCCCCGCGGGGT433900.18198146146170327No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCG404030.16945372176624102No Hit
GCCCGGCTCGCGTCCAGAGTCGCCGCCGCCGCCGGCCCCCCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCC387720.1626131648719327No Hit
CCCCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGGTTCCTCCCGCTCCGGAACCCCCGCGGGGTTGG383360.16078454267333156No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCC352690.14792127596895166No Hit
CCCGGCTCGCGTCCAGAGTCGCCGCCGCCGCCGGCCCCCCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCG341000.1430183875511427No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG340060.14262414331566448No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCAT317380.13311195261284947No Hit
GGGCCCGGCTCGCGTCCAGAGTCGCCGCCGCCGCCGGCCCCCCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGC260760.10936502855670373No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATA241340.10122011041522809No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG30250.041.74196646
GTTGGAC324900.037.50507469
CGTATGC35300.036.259244
TCGTATG38850.033.5674243
ATGCCGT45200.028.62272547
TATCTCG45400.028.3445339
GTATGCC47050.027.2773645
GGTTGGA459700.026.55231368
ACCGCTA21300.025.430162
GTCGGCT48400.024.4497871
CGGGGTT535000.023.0597565
TCGCGGT25050.023.0005262
TGCCGTC58350.021.9948148
GCCGTCT59000.021.86944249
TAGGCGC44400.021.2908510
TCTCGTA59700.021.15061841
ATCTCGT63350.020.2042940
TAGCTTA64400.019.82121834
GCGGGGT629350.019.59722764
CTAGGCG47850.019.4673679
Produced by FastQC (version 0.11.2)