FastQCFastQC Report
Tue 4 Apr 2017
HGVJGBGX2_n01_9w-36_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGVJGBGX2_n01_9w-36_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24103670
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA642870.2667104221058453TruSeq Adapter, Index 15 (97% over 40bp)
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC505340.20965272093419798No Hit
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATAAGCAACATCCGCCGATCCCTGGTCG324160.1344857442870733No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT307090.12740383518360482No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATC293120.12160803728228936No Hit
CTTAAACCTATAACCATCTTTCGGCTAACCTAGCCTCCTCCGTCCCTCCGTACCAACAAGGGGTAGTACAGGAAT283420.11758375384329439No Hit
CTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGGAAGCTGACGAGCGGGAGGCCCTCACGG278700.11562554581937108No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTA273760.11357606538755301No Hit
CAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCT255420.10596726556578315No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT88950.059.22501844
TCGTATG97700.058.6518645
TATGCCG97750.057.95056548
ACGTCTG115550.053.5031415
GTCACAT113150.053.26494229
ATGCCGT107750.052.5726549
AGTCACA116050.052.17138728
GTATGCC111300.051.577447
CACGTCT120650.051.35620514
CAGTCAC119000.050.9939227
ACACGTC121500.050.9685313
GCCGTCT113950.048.32081651
GTCTGAA130850.047.273517
CACATGT128600.047.0534831
ATGTCAG129050.046.5685134
AGAATCT116750.046.54187839
CGTATGC127550.044.95219846
CCGTCTT125500.044.231552
CATGTCA138900.043.34055733
TGCCGTC130600.043.1639350
Produced by FastQC (version 0.11.2)