FastQCFastQC Report
Tue 4 Apr 2017
HGVJGBGX2_n01_9w-16_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGVJGBGX2_n01_9w-16_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15120292
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA411090.2718796700487001TruSeq Adapter, Index 14 (97% over 44bp)
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATAAGCAACATCCGCCGATCCCTGGTCG200430.13255696384699447No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT171320.11330469014751832No Hit
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC158200.10462760904352907No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG60050.055.61137848
CGTATCT57250.055.2601939
CTCGTAT59300.053.4073244
TCGTATG63100.052.59583345
TCCGTAT63950.052.0596237
GTATGCC67900.049.33466347
TTCCGTA68850.048.65506736
ATGCCGT69500.047.90107349
TATCTCG66950.047.0477441
CCGTATC68500.046.38607438
GCCGTCT72150.045.99902751
CGTATGC73900.045.14256346
GTCACAG76300.044.89834229
ACGTCTG78350.044.296315
CAGTCAC77600.044.14603427
CACGTCT81000.042.847114
ACACGTC84400.041.28453413
TGCCGTC81650.040.94225350
GTCTGAA85750.040.51401517
AGTCACA85350.040.2991428
Produced by FastQC (version 0.11.2)