FastQCFastQC Report
Tue 4 Apr 2017
HGVJGBGX2_n01_9s-58_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGVJGBGX2_n01_9s-58_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15023673
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG1435950.955791569744629TruSeq Adapter, Index 12 (100% over 63bp)
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATAAGCAACATCCGCCGATCCCTGGTCG205710.13692390669046112No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT173750.11565081321990968No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTCGAACTGTGAAACTG170330.11337440584602712No Hit
CTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGGAAGCTGACGAGCGGGAGGCCCTCACGG158290.10536038690405469No Hit
CTTGCTTTGAGCACTCTAATTTCTTCAAAGTAACGGCGCCGGAGGCACGACCCGGCCAGTTAAGGCCAGGAGCGC153170.10195243200514283No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT180750.064.4388842
TCGTATG201800.064.0257343
TATGCCG201900.063.8539146
TGTAATC187650.061.92151636
TAATCTC188200.061.7603338
GTATGCC209050.061.73699645
ATGCCGT208950.061.71598447
ACGTCTG215800.061.26009815
CACGTCT218050.060.6914614
TTGTAAT211750.060.68972435
ACACGTC219050.060.60338613
CAGTCAC215850.060.50969727
CTTGTAA215950.059.89139634
CGTATGC219600.058.88218344
GTCTGAA225400.058.78891817
AGTCACC222950.058.5827228
GCCGTCT219300.058.17459549
CCTTGTA223150.057.9585933
CCGTCTT221600.057.555850
GTAATCT202450.057.3446137
Produced by FastQC (version 0.11.2)