FastQCFastQC Report
Tue 4 Apr 2017
HGVJGBGX2_n01_9s-36_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGVJGBGX2_n01_9s-36_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16495183
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG3045261.8461510854411254TruSeq Adapter, Index 11 (100% over 63bp)
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC440090.2667991012891461No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATC300790.18235020490527445No Hit
TTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTA254230.15412378268249585No Hit
CAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATCT239460.14516965346792454No Hit
CCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCAATC233390.14148979129240338No Hit
CGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTACTATCTAACCTCTGTAGGAC204820.12416958332623532No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT183930.11150528005660805No Hit
CTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGGAAGCTGACGAGCGGGAGGCCCTCACGG183810.11143253154572458No Hit
GATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATCA181790.11020793161251985No Hit
TTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCCTAC168620.10222378254306122No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT384850.065.3767642
TCGTATG401050.065.316643
TATGCCG401450.065.2245546
GTATGCC404400.064.73208645
CACGGCT413000.063.89369631
ATGCCGT409350.063.89013747
AGTCACG416450.063.4551228
ACGTCTG421550.063.36041615
CAGTCAC421200.062.927927
GTCACGG420650.062.78893729
GCTACAT416650.062.73761435
CACGTCT427000.062.60019314
ACACGTC428500.062.54189713
GCCGTCT417650.062.3236449
CCGTCTT417400.062.30386450
GTCTGAA431550.061.82825517
CGTATGC425100.061.62924244
CTACATC407900.061.58860836
TACATCT408150.061.39065637
GGCTACA429300.061.31467434
Produced by FastQC (version 0.11.2)