Basic Statistics
Measure | Value |
---|---|
Filename | HGVJGBGX2_n01_9s-16_dr.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 15356149 |
Sequences flagged as poor quality | 0 |
Sequence length | 75 |
%GC | 53 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG | 150840 | 0.9822775228346639 | TruSeq Adapter, Index 10 (100% over 63bp) |
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC | 25737 | 0.16760061392996384 | No Hit |
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATAAGCAACATCCGCCGATCCCTGGTCG | 21236 | 0.13828987983901433 | No Hit |
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT | 17441 | 0.11357665258392582 | No Hit |
CTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGGAAGCTGACGAGCGGGAGGCCCTCACGG | 16539 | 0.10770278407691929 | No Hit |
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTCGAACTGTGAAACTG | 15359 | 0.1000185658526757 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CTCGTAT | 20760 | 0.0 | 64.46243 | 42 |
TATGCCG | 22120 | 0.0 | 64.13101 | 46 |
TCGTATG | 21910 | 0.0 | 64.102005 | 43 |
ATGCCGT | 22550 | 0.0 | 62.571342 | 47 |
GTATGCC | 22715 | 0.0 | 62.5431 | 45 |
CTTATCT | 22030 | 0.0 | 61.841675 | 37 |
AGTCACT | 24180 | 0.0 | 61.677834 | 28 |
TATCTCG | 21955 | 0.0 | 61.488247 | 39 |
ACGTCTG | 24475 | 0.0 | 61.45835 | 15 |
TTATCTC | 22105 | 0.0 | 61.351524 | 38 |
CAGTCAC | 24475 | 0.0 | 60.906223 | 27 |
CACGTCT | 24855 | 0.0 | 60.546295 | 14 |
GCCGTCT | 22805 | 0.0 | 60.480347 | 49 |
ACACGTC | 25040 | 0.0 | 60.250526 | 13 |
GTCTGAA | 25385 | 0.0 | 59.268982 | 17 |
CGTATGC | 23930 | 0.0 | 59.19556 | 44 |
TGCCGTC | 23760 | 0.0 | 58.978294 | 48 |
CCGTCTT | 23455 | 0.0 | 58.923286 | 50 |
CTGAACT | 26350 | 0.0 | 57.05783 | 19 |
AAAAAGG | 22100 | 0.0 | 56.041454 | 69 |