FastQCFastQC Report
Tue 4 Apr 2017
HGVJGBGX2_n01_9s-16_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGVJGBGX2_n01_9s-16_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15356149
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG1508400.9822775228346639TruSeq Adapter, Index 10 (100% over 63bp)
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC257370.16760061392996384No Hit
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATAAGCAACATCCGCCGATCCCTGGTCG212360.13828987983901433No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT174410.11357665258392582No Hit
CTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGGAAGCTGACGAGCGGGAGGCCCTCACGG165390.10770278407691929No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTCGAACTGTGAAACTG153590.1000185658526757No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT207600.064.4624342
TATGCCG221200.064.1310146
TCGTATG219100.064.10200543
ATGCCGT225500.062.57134247
GTATGCC227150.062.543145
CTTATCT220300.061.84167537
AGTCACT241800.061.67783428
TATCTCG219550.061.48824739
ACGTCTG244750.061.4583515
TTATCTC221050.061.35152438
CAGTCAC244750.060.90622327
CACGTCT248550.060.54629514
GCCGTCT228050.060.48034749
ACACGTC250400.060.25052613
GTCTGAA253850.059.26898217
CGTATGC239300.059.1955644
TGCCGTC237600.058.97829448
CCGTCTT234550.058.92328650
CTGAACT263500.057.0578319
AAAAAGG221000.056.04145469