Basic Statistics
Measure | Value |
---|---|
Filename | HGVJGBGX2_n01_9s-15_dr.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 16202546 |
Sequences flagged as poor quality | 0 |
Sequence length | 75 |
%GC | 53 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG | 187265 | 1.155775147930455 | TruSeq Adapter, Index 9 (100% over 63bp) |
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATAAGCAACATCCGCCGATCCCTGGTCG | 24314 | 0.1500628358037064 | No Hit |
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC | 20018 | 0.1235484842937647 | No Hit |
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTCGAACTGTGAAACTG | 17851 | 0.11017404301768377 | No Hit |
CTTGCTTTGAGCACTCTAATTTCTTCAAAGTAACGGCGCCGGAGGCACGACCCGGCCAGTTAAGGCCAGGAGCGC | 16278 | 0.10046569224367578 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TCGTATG | 26210 | 0.0 | 63.563095 | 43 |
CTCGTAT | 23960 | 0.0 | 63.153976 | 42 |
TATGCCG | 26305 | 0.0 | 63.12155 | 46 |
GTATGCC | 27025 | 0.0 | 61.47911 | 45 |
ATGCCGT | 27085 | 0.0 | 61.36726 | 47 |
AGTCACG | 27380 | 0.0 | 61.33606 | 28 |
CGTATGC | 27240 | 0.0 | 61.210495 | 44 |
ACGTCTG | 27955 | 0.0 | 60.56976 | 15 |
GTCACGA | 27820 | 0.0 | 60.390965 | 29 |
CAGTCAC | 27930 | 0.0 | 60.09136 | 27 |
CACGTCT | 28245 | 0.0 | 60.045597 | 14 |
ACACGTC | 28605 | 0.0 | 59.446693 | 13 |
GCCGTCT | 28170 | 0.0 | 58.77149 | 49 |
CCGTCTT | 28165 | 0.0 | 58.769855 | 50 |
CACGATC | 28770 | 0.0 | 58.34922 | 31 |
GTCTGAA | 29260 | 0.0 | 57.927475 | 17 |
TCAGATC | 26305 | 0.0 | 57.261005 | 36 |
GCTTGAA | 29485 | 0.0 | 56.26068 | 59 |
AGATCTC | 26950 | 0.0 | 56.019093 | 38 |
AAAAAGG | 26955 | 0.0 | 55.430866 | 69 |