FastQCFastQC Report
Tue 4 Apr 2017
HGVJGBGX2_n01_9s-15_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGVJGBGX2_n01_9s-15_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16202546
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG1872651.155775147930455TruSeq Adapter, Index 9 (100% over 63bp)
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATAAGCAACATCCGCCGATCCCTGGTCG243140.1500628358037064No Hit
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC200180.1235484842937647No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTCGAACTGTGAAACTG178510.11017404301768377No Hit
CTTGCTTTGAGCACTCTAATTTCTTCAAAGTAACGGCGCCGGAGGCACGACCCGGCCAGTTAAGGCCAGGAGCGC162780.10046569224367578No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG262100.063.56309543
CTCGTAT239600.063.15397642
TATGCCG263050.063.1215546
GTATGCC270250.061.4791145
ATGCCGT270850.061.3672647
AGTCACG273800.061.3360628
CGTATGC272400.061.21049544
ACGTCTG279550.060.5697615
GTCACGA278200.060.39096529
CAGTCAC279300.060.0913627
CACGTCT282450.060.04559714
ACACGTC286050.059.44669313
GCCGTCT281700.058.7714949
CCGTCTT281650.058.76985550
CACGATC287700.058.3492231
GTCTGAA292600.057.92747517
TCAGATC263050.057.26100536
GCTTGAA294850.056.2606859
AGATCTC269500.056.01909338
AAAAAGG269550.055.43086669