FastQCFastQC Report
Tue 4 Apr 2017
HGVJGBGX2_n01_4w-9_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGVJGBGX2_n01_4w-9_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70440965
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAAAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG834350.11844670214270915RNA PCR Primer, Index 28 (100% over 43bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG153950.045.53668643
CTCGTAT146400.044.93947242
ACGTCTG174500.042.9216315
TATGCCG173550.040.3725446
CACGTCT206950.036.458214
CAGTCAC205450.035.1281127
ACACGTC230650.033.02611513
GTATGCC219500.031.98438845
AGTCACC228500.031.22220628
ATGCCGT229900.030.70194847
CGTATGC238150.029.4220644
GTCTGAA261600.028.9868617
CCGTCTT278100.025.26964650
TGTTACC152850.025.12126713
AGCACAC307300.024.9455310
CCAGTCA293100.024.81155826
CGCTATG199900.024.62693629
TTTTTCC332700.024.2211041
CACACGT314750.024.03704312
GCCGTCT294650.023.9553649
Produced by FastQC (version 0.11.2)