FastQCFastQC Report
Tue 4 Apr 2017
HGVJGBGX2_n01_4w-58_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGVJGBGX2_n01_4w-58_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25823777
Sequences flagged as poor quality0
Sequence length75
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC424350.16432530376946794No Hit
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATAAGCAACATCCGCCGATCCCTGGTCG403580.156282328491297No Hit
CTTGCTTTGAGCACTCTAATTTCTTCAAAGTAACGGCGCCGGAGGCACGACCCGGCCAGTTAAGGCCAGGAGCGC296020.11463079161502983No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT293160.11352328514918635No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG273030.10572814348574959TruSeq Adapter, Index 8 (100% over 63bp)
GTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGCGGGATACTTAACGCGTTAGCTACAGCACTGC269830.10448897541207856No Hit
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTA260350.10081793999382815No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTCGAACTGTGAAACTG259320.10041908277011531No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT44700.054.33541542
TATGCCG52650.052.223146
TCGTATG52750.052.06051343
ACGTCTG65200.046.19325615
CACGTCT66550.045.56724514
GTCACAC66000.044.79629529
AGTCACA66500.044.5630628
ATGCCGT61800.044.54668847
CAGTCAC68500.043.31223327
GTATGCC65150.042.78629345
ACACGTC73850.041.43668713
CACACTT71200.041.33100531
TTTTTCC123500.040.686721
TCACACT73650.040.23700330
GTCTGAA78700.038.48879217
TGTTACC72850.037.55394713
CTTGAAT77200.037.53773535
TCTAGTT82100.036.348937
TTTTCCA143900.035.487132
TAGTTGT78700.035.4197279