Basic Statistics
Measure | Value |
---|---|
Filename | HGVJGBGX2_n01_4w-58_dr.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 25823777 |
Sequences flagged as poor quality | 0 |
Sequence length | 75 |
%GC | 54 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC | 42435 | 0.16432530376946794 | No Hit |
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATAAGCAACATCCGCCGATCCCTGGTCG | 40358 | 0.156282328491297 | No Hit |
CTTGCTTTGAGCACTCTAATTTCTTCAAAGTAACGGCGCCGGAGGCACGACCCGGCCAGTTAAGGCCAGGAGCGC | 29602 | 0.11463079161502983 | No Hit |
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT | 29316 | 0.11352328514918635 | No Hit |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG | 27303 | 0.10572814348574959 | TruSeq Adapter, Index 8 (100% over 63bp) |
GTCAATTCCTTTGAGTTTCATTCTTGCGAACGTACTCCCCAGGCGGGATACTTAACGCGTTAGCTACAGCACTGC | 26983 | 0.10448897541207856 | No Hit |
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTA | 26035 | 0.10081793999382815 | No Hit |
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTCGAACTGTGAAACTG | 25932 | 0.10041908277011531 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CTCGTAT | 4470 | 0.0 | 54.335415 | 42 |
TATGCCG | 5265 | 0.0 | 52.2231 | 46 |
TCGTATG | 5275 | 0.0 | 52.060513 | 43 |
ACGTCTG | 6520 | 0.0 | 46.193256 | 15 |
CACGTCT | 6655 | 0.0 | 45.567245 | 14 |
GTCACAC | 6600 | 0.0 | 44.796295 | 29 |
AGTCACA | 6650 | 0.0 | 44.56306 | 28 |
ATGCCGT | 6180 | 0.0 | 44.546688 | 47 |
CAGTCAC | 6850 | 0.0 | 43.312233 | 27 |
GTATGCC | 6515 | 0.0 | 42.786293 | 45 |
ACACGTC | 7385 | 0.0 | 41.436687 | 13 |
CACACTT | 7120 | 0.0 | 41.331005 | 31 |
TTTTTCC | 12350 | 0.0 | 40.68672 | 1 |
TCACACT | 7365 | 0.0 | 40.237003 | 30 |
GTCTGAA | 7870 | 0.0 | 38.488792 | 17 |
TGTTACC | 7285 | 0.0 | 37.553947 | 13 |
CTTGAAT | 7720 | 0.0 | 37.537735 | 35 |
TCTAGTT | 8210 | 0.0 | 36.34893 | 7 |
TTTTCCA | 14390 | 0.0 | 35.48713 | 2 |
TAGTTGT | 7870 | 0.0 | 35.419727 | 9 |