FastQCFastQC Report
Tue 4 Apr 2017
HGVJGBGX2_n01_4w-36_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGVJGBGX2_n01_4w-36_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15611100
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC253620.16246132559524953No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG250090.16020011402143347TruSeq Adapter, Index 7 (100% over 63bp)
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT200790.12862002037012124No Hit
CTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGGAAGCTGACGAGCGGGAGGCCCTCACGG182130.11666698695159214No Hit
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATAAGCAACATCCGCCGATCCCTGGTCG166800.10684705113669121No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT39800.055.39050742
TCGTATG44200.053.46683543
TATGCCG44150.053.4471946
ACGTCTG51150.048.9667815
GTATGCC49400.048.32636645
CACGTCT54000.046.3824214
CAGTCAC53400.045.8682727
ATGCCGT52250.045.69009447
ACACGTC55600.045.4820213
GTCTGAA61500.040.8378317
GCCGTCT58650.039.58720449
AGTCACC63700.038.50573728
TGTTACC45550.037.49102813
CCGTCTT62550.037.39530650
TTTTTCC83850.037.1269531
TAGTTGT47750.035.1856969
TTACCTC50500.033.95279715
CATCTCG65600.033.92202439
TGCCGTC71850.033.6586248
CGTATGC71250.033.55513444