Basic Statistics
Measure | Value |
---|---|
Filename | HGVJGBGX2_n01_4w-36_dr.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 15611100 |
Sequences flagged as poor quality | 0 |
Sequence length | 75 |
%GC | 53 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC | 25362 | 0.16246132559524953 | No Hit |
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG | 25009 | 0.16020011402143347 | TruSeq Adapter, Index 7 (100% over 63bp) |
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT | 20079 | 0.12862002037012124 | No Hit |
CTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGGAAGCTGACGAGCGGGAGGCCCTCACGG | 18213 | 0.11666698695159214 | No Hit |
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATAAGCAACATCCGCCGATCCCTGGTCG | 16680 | 0.10684705113669121 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CTCGTAT | 3980 | 0.0 | 55.390507 | 42 |
TCGTATG | 4420 | 0.0 | 53.466835 | 43 |
TATGCCG | 4415 | 0.0 | 53.44719 | 46 |
ACGTCTG | 5115 | 0.0 | 48.96678 | 15 |
GTATGCC | 4940 | 0.0 | 48.326366 | 45 |
CACGTCT | 5400 | 0.0 | 46.38242 | 14 |
CAGTCAC | 5340 | 0.0 | 45.86827 | 27 |
ATGCCGT | 5225 | 0.0 | 45.690094 | 47 |
ACACGTC | 5560 | 0.0 | 45.48202 | 13 |
GTCTGAA | 6150 | 0.0 | 40.83783 | 17 |
GCCGTCT | 5865 | 0.0 | 39.587204 | 49 |
AGTCACC | 6370 | 0.0 | 38.505737 | 28 |
TGTTACC | 4555 | 0.0 | 37.491028 | 13 |
CCGTCTT | 6255 | 0.0 | 37.395306 | 50 |
TTTTTCC | 8385 | 0.0 | 37.126953 | 1 |
TAGTTGT | 4775 | 0.0 | 35.185696 | 9 |
TTACCTC | 5050 | 0.0 | 33.952797 | 15 |
CATCTCG | 6560 | 0.0 | 33.922024 | 39 |
TGCCGTC | 7185 | 0.0 | 33.65862 | 48 |
CGTATGC | 7125 | 0.0 | 33.555134 | 44 |