FastQCFastQC Report
Tue 4 Apr 2017
HGVJGBGX2_n01_4w-1_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGVJGBGX2_n01_4w-1_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55280716
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC618010.11179486170186365No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG118550.043.4192143
ACGTCTG129500.042.7312215
CTCGTAT103800.041.612442
TATGCCG125800.041.32700346
CACGTCT157100.035.57547814
ACACGTC159550.035.00750413
CAGTCAC160450.032.939627
CGTATGC160800.032.16087744
GTATGCC162100.032.1580645
CTAATCT139350.031.14508237
ATGCCGT171350.030.70349547
GTCTGAA187950.030.06640417
TAATCTC152000.028.66661338
TGTTACC131200.028.34627713
TTACCTC136150.027.11295315
AGTCACC195450.026.8115228
CTTAAAC159800.026.1751631
CGCTATG166600.025.75993229
GTCGCTA169050.025.50904327
GCCGTCT206900.025.09472349
Produced by FastQC (version 0.11.2)