FastQCFastQC Report
Tue 4 Apr 2017
HGVJGBGX2_n01_4w-16_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGVJGBGX2_n01_4w-16_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13957937
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG431270.3089783253786TruSeq Adapter, Index 6 (100% over 63bp)
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC202760.14526502018170737No Hit
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATAAGCAACATCCGCCGATCCCTGGTCG155770.11159958667244307No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT59000.058.24070742
TATGCCG62850.056.7018546
TCGTATG63700.055.8391243
GTCACGC67100.053.21328429
TATCTCG66500.051.4648739
ACGTCTG72000.050.9828115
AGTCACG71650.050.1227828
GTATGCC71150.049.94201745
CACGTCT74500.049.22549414
GCCAATA73000.048.77105734
CGCCAAT73750.048.1811833
ATGCCGT74650.047.96948647
ACACGTC77250.047.8302413
TCACGCC75050.047.7601330
CAGTCAC76000.047.526127
CACGCCA75950.047.14892631
CCAATAT78350.045.66145735
ATATCTC76500.045.05328438
CGTATGC79450.044.55177344
ACGCCAA81400.044.07691232
Produced by FastQC (version 0.11.2)