FastQCFastQC Report
Tue 4 Apr 2017
HGVJGBGX2_n01_4w-15_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGVJGBGX2_n01_4w-15_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20708974
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG353690.17079069199661942TruSeq Adapter, Index 5 (100% over 63bp)
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATAAGCAACATCCGCCGATCCCTGGTCG288400.139263297158034No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTCGAACTGTGAAACTG252390.12187470031108252No Hit
CTTGCTTTGAGCACTCTAATTTCTTCAAAGTAACGGCGCCGGAGGCACGACCCGGCCAGTTAAGGCCAGGAGCGC247030.1192864504055102No Hit
CTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGGAAGCTGACGAGCGGGAGGCCCTCACGG244280.11795852368156916No Hit
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTA241770.1167464887444448No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT225970.10911694611234725No Hit
CTTAAACCTATAACCATCTTTCGGCTAACCTAGCCTCCTCCGTCCCTCCGTACCAACAAGGGGTAGTACAGGAAT209700.10126044873106702No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG59450.055.36013846
TCGTATG59850.054.8771143
CTCGTAT52400.054.64699642
ATGCCGT66400.049.7211747
ACGTCTG70200.049.63559715
CACAGTG69250.048.87060533
CAGTCAC70500.048.24831427
ACACAGT70450.048.18496732
AGTCACA70750.047.78537828
GTCACAC72000.046.81202729
CACGTCT74650.046.76917314
GTATGCC71800.046.1748945
ACACGTC75950.046.1503413
GTCTGAA76550.045.60845617
CACACAG75550.045.29765731
GCCGTCT73450.044.29194349
CGTATGC81000.040.84574544
ACAGTGA85800.039.44393534
CCGTCTT85500.038.25146550
TGCCGTC89400.037.35429448