Basic Statistics
Measure | Value |
---|---|
Filename | HGVJGBGX2_n01_4w-15_dr.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 20708974 |
Sequences flagged as poor quality | 0 |
Sequence length | 75 |
%GC | 53 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG | 35369 | 0.17079069199661942 | TruSeq Adapter, Index 5 (100% over 63bp) |
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATAAGCAACATCCGCCGATCCCTGGTCG | 28840 | 0.139263297158034 | No Hit |
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTCGAACTGTGAAACTG | 25239 | 0.12187470031108252 | No Hit |
CTTGCTTTGAGCACTCTAATTTCTTCAAAGTAACGGCGCCGGAGGCACGACCCGGCCAGTTAAGGCCAGGAGCGC | 24703 | 0.1192864504055102 | No Hit |
CTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGGAAGCTGACGAGCGGGAGGCCCTCACGG | 24428 | 0.11795852368156916 | No Hit |
GCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAACTAGCTA | 24177 | 0.1167464887444448 | No Hit |
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT | 22597 | 0.10911694611234725 | No Hit |
CTTAAACCTATAACCATCTTTCGGCTAACCTAGCCTCCTCCGTCCCTCCGTACCAACAAGGGGTAGTACAGGAAT | 20970 | 0.10126044873106702 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TATGCCG | 5945 | 0.0 | 55.360138 | 46 |
TCGTATG | 5985 | 0.0 | 54.87711 | 43 |
CTCGTAT | 5240 | 0.0 | 54.646996 | 42 |
ATGCCGT | 6640 | 0.0 | 49.72117 | 47 |
ACGTCTG | 7020 | 0.0 | 49.635597 | 15 |
CACAGTG | 6925 | 0.0 | 48.870605 | 33 |
CAGTCAC | 7050 | 0.0 | 48.248314 | 27 |
ACACAGT | 7045 | 0.0 | 48.184967 | 32 |
AGTCACA | 7075 | 0.0 | 47.785378 | 28 |
GTCACAC | 7200 | 0.0 | 46.812027 | 29 |
CACGTCT | 7465 | 0.0 | 46.769173 | 14 |
GTATGCC | 7180 | 0.0 | 46.17489 | 45 |
ACACGTC | 7595 | 0.0 | 46.15034 | 13 |
GTCTGAA | 7655 | 0.0 | 45.608456 | 17 |
CACACAG | 7555 | 0.0 | 45.297657 | 31 |
GCCGTCT | 7345 | 0.0 | 44.291943 | 49 |
CGTATGC | 8100 | 0.0 | 40.845745 | 44 |
ACAGTGA | 8580 | 0.0 | 39.443935 | 34 |
CCGTCTT | 8550 | 0.0 | 38.251465 | 50 |
TGCCGTC | 8940 | 0.0 | 37.354294 | 48 |