FastQCFastQC Report
Tue 4 Apr 2017
HGVJGBGX2_n01_4w-11_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGVJGBGX2_n01_4w-11_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60348398
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACGATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG703700.11660624363218391RNA PCR Primer, Index 31 (100% over 43bp)
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC623580.10333000057433173No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG146100.042.57574543
CTCGTAT143250.042.2668242
ACGTCTG162750.040.9963315
ACGATAT172300.036.2410435
TATCTCG173750.034.8872639
CCACGAT182150.034.69738833
CACGTCT194550.034.64996714
TATGCCG179600.034.59459346
CAGTCAC187850.033.79147327
ACACGTC203650.033.3387913
CGATATC192500.031.66782436
GTATGCC199700.031.30300545
CGTATGC202500.030.66629644
ATGCCGT204650.030.59605447
CACGATA214400.029.81626934
GTCTGAA232300.029.22710217
AGTCACC227450.027.9537428
ACCACGA235750.027.14515532
ATATCTC230050.026.604238
GATATCT238500.025.69044537
Produced by FastQC (version 0.11.2)