Basic Statistics
Measure | Value |
---|---|
Filename | HGVJGBGX2_n01_4s-58_dr.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 15488381 |
Sequences flagged as poor quality | 0 |
Sequence length | 75 |
%GC | 53 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG | 25212 | 0.16278008656940968 | TruSeq Adapter, Index 4 (100% over 63bp) |
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATAAGCAACATCCGCCGATCCCTGGTCG | 23560 | 0.15211402663712884 | No Hit |
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC | 22528 | 0.14545096740582505 | No Hit |
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT | 16220 | 0.10472366349975508 | No Hit |
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTCGAACTGTGAAACTG | 15881 | 0.10253492601970471 | No Hit |
CTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGGAAGCTGACGAGCGGGAGGCCCTCACGG | 15643 | 0.100998290266749 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TATGCCG | 4520 | 0.0 | 55.029934 | 46 |
CTCGTAT | 3960 | 0.0 | 54.18903 | 42 |
TCGTATG | 4645 | 0.0 | 53.921787 | 43 |
ACTGACC | 4855 | 0.0 | 51.517044 | 32 |
AGTCACT | 5090 | 0.0 | 49.138397 | 28 |
ACGTCTG | 5245 | 0.0 | 48.87146 | 15 |
ATGCCGT | 5255 | 0.0 | 47.333225 | 47 |
GTATGCC | 5310 | 0.0 | 46.972897 | 45 |
CACGTCT | 5525 | 0.0 | 46.70693 | 14 |
ACACGTC | 5680 | 0.0 | 45.614567 | 13 |
CAGTCAC | 5680 | 0.0 | 44.39865 | 27 |
CTGACCA | 5885 | 0.0 | 42.383224 | 33 |
GTCTGAA | 6285 | 0.0 | 40.94921 | 17 |
GCCGTCT | 6185 | 0.0 | 39.825962 | 49 |
CCGTCTT | 6385 | 0.0 | 38.90292 | 50 |
TGTTACC | 4280 | 0.0 | 36.75641 | 13 |
TGCCGTC | 6905 | 0.0 | 36.22269 | 48 |
CGTATGC | 6955 | 0.0 | 36.11167 | 44 |
ACCAATC | 5970 | 0.0 | 36.00227 | 36 |
CAATCTC | 5995 | 0.0 | 35.564743 | 38 |