FastQCFastQC Report
Tue 4 Apr 2017
HGVJGBGX2_n01_4s-58_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGVJGBGX2_n01_4s-58_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15488381
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG252120.16278008656940968TruSeq Adapter, Index 4 (100% over 63bp)
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATAAGCAACATCCGCCGATCCCTGGTCG235600.15211402663712884No Hit
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC225280.14545096740582505No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT162200.10472366349975508No Hit
GCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGCAAGTATGAACTAATTCGAACTGTGAAACTG158810.10253492601970471No Hit
CTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGGAAGCTGACGAGCGGGAGGCCCTCACGG156430.100998290266749No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG45200.055.02993446
CTCGTAT39600.054.1890342
TCGTATG46450.053.92178743
ACTGACC48550.051.51704432
AGTCACT50900.049.13839728
ACGTCTG52450.048.8714615
ATGCCGT52550.047.33322547
GTATGCC53100.046.97289745
CACGTCT55250.046.7069314
ACACGTC56800.045.61456713
CAGTCAC56800.044.3986527
CTGACCA58850.042.38322433
GTCTGAA62850.040.9492117
GCCGTCT61850.039.82596249
CCGTCTT63850.038.9029250
TGTTACC42800.036.7564113
TGCCGTC69050.036.2226948
CGTATGC69550.036.1116744
ACCAATC59700.036.0022736
CAATCTC59950.035.56474338