FastQCFastQC Report
Tue 4 Apr 2017
HGVJGBGX2_n01_4s-36_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGVJGBGX2_n01_4s-36_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15264840
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG277670.1819016773185962TruSeq Adapter, Index 3 (100% over 63bp)
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC243110.15926141381108483No Hit
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATAAGCAACATCCGCCGATCCCTGGTCG211660.13865851197916257No Hit
TGATCCAAGTATGAGTGTAGGGTAGTAACAGCACCTGATAGTCCTACAGAGGTTAGATAGTAGGACTGTTTGATC167990.11005028549267468No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT42150.051.31887442
TCGTATG45500.050.80080443
ACTTAGG46600.050.41500532
TATGCCG46950.049.67194446
ACGTCTG54050.045.6373515
GTATGCC51500.045.3505945
AGTCACT53150.044.9809528
CACGTCT56850.043.6323314
GTCACTT54450.043.59023329
TTAGGCA55750.042.14061734
ACACGTC59950.041.43366213
ATGCCGT57600.040.7273847
CAGTCAC60600.039.67882527
GTCTGAA67750.036.6126417
GCCGTCT64600.035.460149
CCGTCTT65600.034.7095350
TGTTACC48200.034.6424313
TTTTTCC88200.034.1619031
CGTATGC68600.033.9961544
TTACCTC50050.033.2930115
Produced by FastQC (version 0.11.2)