FastQCFastQC Report
Tue 4 Apr 2017
HGVJGBGX2_n01_4s-30_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGVJGBGX2_n01_4s-30_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61733875
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC672540.10894180869093345No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT106500.034.17615542
ACGTCTG135300.032.1278615
TCGTATG120500.031.8087843
CGTATGC122450.031.4991544
TATGCCG135100.028.98292546
TGTTACC175700.027.07706513
CACGTCT165950.026.48512314
ACACGTC171550.026.1433813
AGTCACG151900.025.93629628
TTACCTC185550.025.26780915
TCTAGTT216750.024.4639347
CTCTAGT228500.023.6473356
ACTCTAG228600.023.6068025
TCTAATG198850.023.3692320
TAGTTGT212500.023.329589
CTAATGC200500.022.95319621
CAGTCAC179200.022.4470627
CACACGT201200.022.08472812
GTATGCC179850.021.9060545
TACTCTA234950.020.9420744
Produced by FastQC (version 0.11.2)