FastQCFastQC Report
Tue 4 Apr 2017
HGVJGBGX2_n01_4s-18_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGVJGBGX2_n01_4s-18_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28337703
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC335050.11823470660271936No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA321640.11350249524458633TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACGT64550.041.098829
CTCGTAT62150.039.46744544
TCGTATG67350.038.46883845
ACGTCCG69450.038.14956732
ACGTCTG74800.037.77432615
TATGCCG71700.036.47150848
TCACGTC74750.035.53688430
CGTATGC76900.034.0499246
CGCACAT77750.033.67885637
CCGCACA80700.032.96055236
AGTCACG82500.032.32390628
CACGTCT88400.032.31424314
ACACGTC89550.031.9762613
GTATGCC89300.029.59219447
CAGTCAC91900.029.16786227
TGTTACC75600.028.38466313
GTCCGCA94650.028.10196534
GTCGCTA79500.027.07799727
TTACCTC79100.026.99781215
CGCTATG79150.026.89263329
Produced by FastQC (version 0.11.2)