FastQCFastQC Report
Tue 4 Apr 2017
HGVJGBGX2_n01_4s-16_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGVJGBGX2_n01_4s-16_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14910407
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG402720.27009323085546894TruSeq Adapter, Index 2 (100% over 63bp)
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC224420.15051232337252768No Hit
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATAAGCAACATCCGCCGATCCCTGGTCG188370.12633457959933622No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT152790.10247205190307683No Hit
CTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGGAAGCTGACGAGCGGGAGGCCCTCACGG152720.10242510482778908No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT59100.056.97360242
TATGCCG63700.056.2158846
TCGTATG63350.056.14639343
ACGTCTG72200.051.5584615
GTATGCC70200.050.66721745
TATCTCG68300.049.55215539
CACCGAT73950.049.2633531
CAGTCAC76250.048.36554327
CACGTCT77900.048.09589414
ATGCCGT74700.047.8452547
ACACGTC79250.047.53779613
CGATGTA78350.046.23277734
TGTATCT75250.045.250237
GTCTGAA84050.044.4123517
AGTCACC83050.044.23929228
CCGATGT82600.043.89558433
GCCGTCT82450.043.09706549
CCGTCTT82500.043.07180850
CGTATGC85650.041.6082944
TGCCGTC93450.038.50376548