Basic Statistics
Measure | Value |
---|---|
Filename | HGVJGBGX2_n01_4s-16_dr.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 14910407 |
Sequences flagged as poor quality | 0 |
Sequence length | 75 |
%GC | 53 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG | 40272 | 0.27009323085546894 | TruSeq Adapter, Index 2 (100% over 63bp) |
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC | 22442 | 0.15051232337252768 | No Hit |
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATAAGCAACATCCGCCGATCCCTGGTCG | 18837 | 0.12633457959933622 | No Hit |
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTATCCCTGTGGTAACT | 15279 | 0.10247205190307683 | No Hit |
CTGACATGCGTGCGAGTCGACGGGTTCTGAAACCTGGGATGCGCAAGGAAGCTGACGAGCGGGAGGCCCTCACGG | 15272 | 0.10242510482778908 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CTCGTAT | 5910 | 0.0 | 56.973602 | 42 |
TATGCCG | 6370 | 0.0 | 56.21588 | 46 |
TCGTATG | 6335 | 0.0 | 56.146393 | 43 |
ACGTCTG | 7220 | 0.0 | 51.55846 | 15 |
GTATGCC | 7020 | 0.0 | 50.667217 | 45 |
TATCTCG | 6830 | 0.0 | 49.552155 | 39 |
CACCGAT | 7395 | 0.0 | 49.26335 | 31 |
CAGTCAC | 7625 | 0.0 | 48.365543 | 27 |
CACGTCT | 7790 | 0.0 | 48.095894 | 14 |
ATGCCGT | 7470 | 0.0 | 47.84525 | 47 |
ACACGTC | 7925 | 0.0 | 47.537796 | 13 |
CGATGTA | 7835 | 0.0 | 46.232777 | 34 |
TGTATCT | 7525 | 0.0 | 45.2502 | 37 |
GTCTGAA | 8405 | 0.0 | 44.41235 | 17 |
AGTCACC | 8305 | 0.0 | 44.239292 | 28 |
CCGATGT | 8260 | 0.0 | 43.895584 | 33 |
GCCGTCT | 8245 | 0.0 | 43.097065 | 49 |
CCGTCTT | 8250 | 0.0 | 43.071808 | 50 |
CGTATGC | 8565 | 0.0 | 41.60829 | 44 |
TGCCGTC | 9345 | 0.0 | 38.503765 | 48 |