FastQCFastQC Report
Tue 4 Apr 2017
HGVJGBGX2_n01_4s-15_dr.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGVJGBGX2_n01_4s-15_dr.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22054358
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG405260.18375506555212354TruSeq Adapter, Index 1 (100% over 63bp)
CCGGAACCCAAAGACTTTGATTTCTCATAAGGTGCCGGCGGAGTCCTATAAGCAACATCCGCCGATCCCTGGTCG244810.11100300448555336No Hit
TTTTTCCACCTTAGAGTACAAACGGAACACGAGAATTTTTACCCTACAGAGTAACCTAGATTGATCAAACAGTCC240740.10915756423288314No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT65750.053.04852342
TATGCCG73700.052.56666646
TCGTATG73950.052.39109443
ACGTCTG85950.046.48133515
GTATGCC84450.046.2027745
CAGTCAC90100.043.41946827
ATGCCGT89650.043.4064347
ACACGTC94650.042.5736713
CACGTCT95500.042.15852414
AGTCACA93050.042.04292328
CGTATGC92950.041.79303744
GTCTGAA103800.038.75378417
CACGATC91700.037.8105236
GCCGTCT103600.037.695149
GTCACAT104700.037.2660329
CCGTCTT106800.036.9214850
TGCCGTC125450.031.48698248
CTGAACT128500.031.46527519
CCAGTCA127200.030.8639426
AGCACAC132450.030.63136510
Produced by FastQC (version 0.11.2)