FastQCFastQC Report
Thu 27 Aug 2020
HGVH7AFX2_n02_vk8-9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHGVH7AFX2_n02_vk8-9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6623422
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG231410.349381331885542No Hit
CGGGAAGAGATTTAGTATGTGGGACAGAATGTCGGCGGCAGTATTGAGAC111960.16903648899315188No Hit
TGGGGTGGTATAGTCCGCATTGGGATGTTACTTTCCTGTTATGGCATGGA97050.14652546674513567No Hit
CGGGAAGGAATAAGAAGCAACTAAACGAGGGTGTAGAAAAAGACGAAGAG96260.14533272981851375No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG107300.033.07541370
CATTAAA261150.029.68570961
TCATTAA265850.028.76593460
CGTATCA267000.028.2631756
CCGTATC269000.028.04002655
CGAGATC27400.027.8451775
GTATCAT263350.027.81757757
ATCATTA282350.026.85060359
TGTCGGC33250.026.63010830
CGCCGTA290450.026.4392253
TATCATT283200.026.36216258
GCCGTAT300000.026.0409254
CTAGATC23600.025.9519565
AAAAGGG213950.025.71622870
TCGCCGT302550.025.36853252
CGAGCAG13350.024.6715151
GTCGCCG326400.024.1903951
GTGGGAC37100.023.9609419
CGCGTAG2051.8189894E-1223.9139352
TTAAAAA347850.023.8563163
Produced by FastQC (version 0.11.5)